Site-directed point mutations in embryonic stem cells: A gene-targeting tag-and-exchange strategy

G. Roger Askew, Thomas C Doetschman, Jerry B. Lingrel

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Sequential gene targeting was used to introduce point mutations into one α2 isoform Na,K-ATPase homolog in mouse embryonic stem (ES) cells. In the first round of targeted replacement, the gene was tagged with selectable markers by insertion of a Neor/HSV-tk gene cassette, and this event was selected for by gain of neomycin (G418) resistance. In the second targeted replacement event, the tagged genomic sequence was exchanged with a vector consisting of homologous genomic sequences carrying five site-directed nucleotide substitutions. Embryonic stem cell clones modified by exchange with the mutation vector were selected for loss of the HSV-tk gene by resistance to ganciclovir. Candidate clones were further screened and identified by polymerase chain reaction and Southern blot analysis. By this strategy, the endogenous α2 isoform Na,K-ATPase gene was altered to encode two other amino acids so that the enzyme is resistant to inhibition by cardiac glycosides while maintaining its transmembrane ion-pumping function. Since the initial tagging event and the subsequent mutation-exchange event are independent of one another, a tagged cell line can be used to generate a variety of mutant lines by exchange with various mutation vectors at the tagged locus. This method should be useful for testing specific mutations introduced into the genomes of tissue culture cells and animals and for developing animal models encompassing the mutational variability of known genetic disorders.

Original languageEnglish (US)
Pages (from-to)4115-4124
Number of pages10
JournalMolecular and Cellular Biology
Volume13
Issue number7
StatePublished - Jul 1993
Externally publishedYes

Fingerprint

Gene Targeting
Embryonic Stem Cells
Point Mutation
Mutation
Genes
Protein Isoforms
Clone Cells
Cardiac Glycosides
Inborn Genetic Diseases
Ganciclovir
Neomycin
Sequence Homology
Southern Blotting
Nucleotides
Animal Models
Cell Culture Techniques
Genome
Ions
Amino Acids
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Site-directed point mutations in embryonic stem cells : A gene-targeting tag-and-exchange strategy. / Askew, G. Roger; Doetschman, Thomas C; Lingrel, Jerry B.

In: Molecular and Cellular Biology, Vol. 13, No. 7, 07.1993, p. 4115-4124.

Research output: Contribution to journalArticle

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