Size-dependent self-assembly of submicron/nano beads-protein conjugates for construction of a protein nanoarray

Tremaine B. Powell, Phat L. Tran, Keesung Kim, Jeong Yeol Yoon

Research output: Contribution to journalArticle

4 Scopus citations


A protein nanoarray is created when submicro and nano beads, varying in their size and each conjugated with different proteins, self-assemble to specific locations depending on the diameter matching the surface electron beam patterns created. Protein binding is confirmed from the fluorescence attenuation of the beads upon antigen-antibody binding on the bead surface. This method, called size-dependent self-assembly, allows control of the location of each type of bead, and thus, control of the location of multiple proteins. It provides fast multi-component patterning with a high binding resolution, which can be detected using a fluorescent light microscope. This method is developed to be a simple stand-alone tool for analysis of protein interactions. In addition, it has the potential to be used in conjunction with other protein analysis methods, such as enzyme-linked immunosorbent assay (ELISA) and atomic force microscopy (AFM).

Original languageEnglish (US)
Pages (from-to)2459-2463
Number of pages5
JournalMaterials Science and Engineering C
Issue number8
StatePublished - Oct 15 2009



  • Electron beam lithography
  • Fluorescent attenuation
  • Fluorescent beads
  • Gold nanoparticles
  • Protein nanoarray
  • Self-assembly

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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