Skeletal muscle satellite cell migration to injured tissue measured with111In-oxine and high-resolution SPECT imaging

Jennifer L. Elster, Christopher R. Rathbone, Zhonglin Liu, Xiasong Liu, Harrison H. Barrett, Robert P. Rhoads, Ronald E. Allen

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The delivery of adult skeletal muscle stem cells, called satellite cells, to several injured muscles via the circulation would be useful, however, an improved understanding of cell fate and biodistribution following their delivery is important for this goal to be achieved. The objective of this study was to evaluate the ability of systemically delivered satellite cells to home to injured skeletal muscle using single-photon emission computed tomography (SPECT) imaging of 111In-labeled satellite cells. Satellite cells labeled with 111In-oxine and green fluorescent protein (GFP) were injected intravenously after bupivicaine-induced injury to the tibialis anterior muscle. Animals were imaged with a high-resolution SPECT system called FastSPECT II for up to 7 days after transplantation. In vivo FastSPECT II imaging demonstrated a three to five-fold greater number of transplanted satellite cells in bupivicaine-injured muscle as compared to un-injured muscle after transplantation; a finding that was verified through autoradiograph analysis and quantification of GFP expression. Satellite cells also accumulated in other organs including the lung, liver, and spleen, as determined by biodistribution measurements. These data support the ability of satellite cells to home to injured muscle and support the use of SPECT and autoradiograph imaging techniques to track systemically transplanted 111In labeled satellite cells in vivo, and suggest their homing may be improved by reducing their entrapment in filter organs.

Original languageEnglish (US)
Pages (from-to)417-427
Number of pages11
JournalJournal of Muscle Research and Cell Motility
Volume34
Issue number5-6
DOIs
StatePublished - Dec 1 2013

Keywords

  • In
  • SPECT
  • Satellite cell
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Cell Biology

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