Skin mucous cell response to acid stress in male and female brown bullhead catfish, Ictalurus nebulosus (Lesueur)

Edwin M. Zuchelkowski, Robert Clark Lantz, David E. Hinton

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Exposure of brown bullhead catfish Ictalurus nebulosus (Lesueur) to water made acid in pH by addition of dilute sulfuric acid, removed a sexual dimorphism observed in mucosubstance-containing cells of control epidermis. When stained by the alcian blue-periodic acid Schiff's reagent technique for acidic and neutral mucosubstances, positive cells in control males are seen primarily at the external surface. A few mucosubstance-containing cells are observed in basal regions. By contrast, skin of control female brown bullheads appears to contain far more mucosubstance. Morphometric analysis of skin reacted by the above technique for mucosubstance revealed the following: 1) mucous cells of control females are more than twice as large as those of control males; 2) 48 h after initiation of acid stress (pH 5.7-3.8) both sexes respond with hyperplasia of mucous cells; 3) more severe acid stress (pH 5.2-3.8) leads to hypertrophy of mucous cells in epidermis of males. Preliminary observations concerning development of the response indicate that an initial increase in mucosubstance occurs throughout the entire epidermis by 48 h. This increase is followed by movement of mucous cells toward the surface and eventual release of mucosubstance. After 3 or 4 days of exposure, the majority of mucous cells appear within the upper one-third of the epidermis. Endocrine factors possibly mediating mucosubstance in control and acid-stressed epidermis are discussed.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalAquatic Toxicology
Volume8
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Ictalurus
Ictaluridae
skin (animal)
catfish
skin
epidermis (animal)
Epidermis
Skin
Acids
acids
acid
cells
sexual dimorphism
sulfuric acid
Alcian Blue
Periodic Acid
Sex Characteristics
Hypertrophy
Cell Movement
Hyperplasia

Keywords

  • acid stress
  • catfish
  • dimorphism
  • morphometry
  • mucous
  • skin

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Skin mucous cell response to acid stress in male and female brown bullhead catfish, Ictalurus nebulosus (Lesueur). / Zuchelkowski, Edwin M.; Lantz, Robert Clark; Hinton, David E.

In: Aquatic Toxicology, Vol. 8, No. 3, 1986, p. 139-148.

Research output: Contribution to journalArticle

@article{cb52d48e095c41ef9987d7277fabdc3a,
title = "Skin mucous cell response to acid stress in male and female brown bullhead catfish, Ictalurus nebulosus (Lesueur)",
abstract = "Exposure of brown bullhead catfish Ictalurus nebulosus (Lesueur) to water made acid in pH by addition of dilute sulfuric acid, removed a sexual dimorphism observed in mucosubstance-containing cells of control epidermis. When stained by the alcian blue-periodic acid Schiff's reagent technique for acidic and neutral mucosubstances, positive cells in control males are seen primarily at the external surface. A few mucosubstance-containing cells are observed in basal regions. By contrast, skin of control female brown bullheads appears to contain far more mucosubstance. Morphometric analysis of skin reacted by the above technique for mucosubstance revealed the following: 1) mucous cells of control females are more than twice as large as those of control males; 2) 48 h after initiation of acid stress (pH 5.7-3.8) both sexes respond with hyperplasia of mucous cells; 3) more severe acid stress (pH 5.2-3.8) leads to hypertrophy of mucous cells in epidermis of males. Preliminary observations concerning development of the response indicate that an initial increase in mucosubstance occurs throughout the entire epidermis by 48 h. This increase is followed by movement of mucous cells toward the surface and eventual release of mucosubstance. After 3 or 4 days of exposure, the majority of mucous cells appear within the upper one-third of the epidermis. Endocrine factors possibly mediating mucosubstance in control and acid-stressed epidermis are discussed.",
keywords = "acid stress, catfish, dimorphism, morphometry, mucous, skin",
author = "Zuchelkowski, {Edwin M.} and Lantz, {Robert Clark} and Hinton, {David E.}",
year = "1986",
doi = "10.1016/0166-445X(86)90060-3",
language = "English (US)",
volume = "8",
pages = "139--148",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Skin mucous cell response to acid stress in male and female brown bullhead catfish, Ictalurus nebulosus (Lesueur)

AU - Zuchelkowski, Edwin M.

AU - Lantz, Robert Clark

AU - Hinton, David E.

PY - 1986

Y1 - 1986

N2 - Exposure of brown bullhead catfish Ictalurus nebulosus (Lesueur) to water made acid in pH by addition of dilute sulfuric acid, removed a sexual dimorphism observed in mucosubstance-containing cells of control epidermis. When stained by the alcian blue-periodic acid Schiff's reagent technique for acidic and neutral mucosubstances, positive cells in control males are seen primarily at the external surface. A few mucosubstance-containing cells are observed in basal regions. By contrast, skin of control female brown bullheads appears to contain far more mucosubstance. Morphometric analysis of skin reacted by the above technique for mucosubstance revealed the following: 1) mucous cells of control females are more than twice as large as those of control males; 2) 48 h after initiation of acid stress (pH 5.7-3.8) both sexes respond with hyperplasia of mucous cells; 3) more severe acid stress (pH 5.2-3.8) leads to hypertrophy of mucous cells in epidermis of males. Preliminary observations concerning development of the response indicate that an initial increase in mucosubstance occurs throughout the entire epidermis by 48 h. This increase is followed by movement of mucous cells toward the surface and eventual release of mucosubstance. After 3 or 4 days of exposure, the majority of mucous cells appear within the upper one-third of the epidermis. Endocrine factors possibly mediating mucosubstance in control and acid-stressed epidermis are discussed.

AB - Exposure of brown bullhead catfish Ictalurus nebulosus (Lesueur) to water made acid in pH by addition of dilute sulfuric acid, removed a sexual dimorphism observed in mucosubstance-containing cells of control epidermis. When stained by the alcian blue-periodic acid Schiff's reagent technique for acidic and neutral mucosubstances, positive cells in control males are seen primarily at the external surface. A few mucosubstance-containing cells are observed in basal regions. By contrast, skin of control female brown bullheads appears to contain far more mucosubstance. Morphometric analysis of skin reacted by the above technique for mucosubstance revealed the following: 1) mucous cells of control females are more than twice as large as those of control males; 2) 48 h after initiation of acid stress (pH 5.7-3.8) both sexes respond with hyperplasia of mucous cells; 3) more severe acid stress (pH 5.2-3.8) leads to hypertrophy of mucous cells in epidermis of males. Preliminary observations concerning development of the response indicate that an initial increase in mucosubstance occurs throughout the entire epidermis by 48 h. This increase is followed by movement of mucous cells toward the surface and eventual release of mucosubstance. After 3 or 4 days of exposure, the majority of mucous cells appear within the upper one-third of the epidermis. Endocrine factors possibly mediating mucosubstance in control and acid-stressed epidermis are discussed.

KW - acid stress

KW - catfish

KW - dimorphism

KW - morphometry

KW - mucous

KW - skin

UR - http://www.scopus.com/inward/record.url?scp=0022486712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022486712&partnerID=8YFLogxK

U2 - 10.1016/0166-445X(86)90060-3

DO - 10.1016/0166-445X(86)90060-3

M3 - Article

VL - 8

SP - 139

EP - 148

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

IS - 3

ER -