Small-Angle Neutron Scattering Reveals Energy Landscape for Rhodopsin Photoactivation

Suchithranga M.D.C. Perera, Udeep Chawla, Utsab R. Shrestha, Debsindhu Bhowmik, Andrey V. Struts, Shuo Qian, Xiang Qiang Chu, Michael F Brown

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Knowledge of the activation principles for G-protein-coupled receptors (GPCRs) is critical to development of new pharmaceuticals. Rhodopsin is the archetype for the largest GPCR family, yet the changes in protein dynamics that trigger signaling are not fully understood. Here we show that rhodopsin can be investigated by small-angle neutron scattering (SANS) in fully protiated detergent micelles under contrast matching to resolve light-induced changes in the protein structure. In SANS studies of membrane proteins, the zwitterionic detergent [(cholamidopropyl)dimethylammonio]-propanesulfonate (CHAPS) is advantageous because of the low contrast difference between the hydrophobic core and hydrophilic head groups as compared with alkyl glycoside detergents. Combining SANS results with quasielastic neutron scattering reveals how changes in volumetric protein shape are coupled (slaved) to the aqueous solvent. Upon light exposure, rhodopsin is swollen by the penetration of water into the protein core, allowing interactions with effector proteins in the visual signaling mechanism.

Original languageEnglish (US)
Pages (from-to)7064-7071
Number of pages8
JournalJournal of Physical Chemistry Letters
Volume9
Issue number24
DOIs
StatePublished - Dec 20 2018

Fingerprint

Rhodopsin
Neutron scattering
neutron scattering
proteins
Proteins
Detergents
detergents
G-Protein-Coupled Receptors
energy
Micelles
Glycosides
effectors
Membrane Proteins
glucosides
Chemical activation
Drug products
Water
micelles
penetration
actuators

ASJC Scopus subject areas

  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Small-Angle Neutron Scattering Reveals Energy Landscape for Rhodopsin Photoactivation. / Perera, Suchithranga M.D.C.; Chawla, Udeep; Shrestha, Utsab R.; Bhowmik, Debsindhu; Struts, Andrey V.; Qian, Shuo; Chu, Xiang Qiang; Brown, Michael F.

In: Journal of Physical Chemistry Letters, Vol. 9, No. 24, 20.12.2018, p. 7064-7071.

Research output: Contribution to journalArticle

Perera, SMDC, Chawla, U, Shrestha, UR, Bhowmik, D, Struts, AV, Qian, S, Chu, XQ & Brown, MF 2018, 'Small-Angle Neutron Scattering Reveals Energy Landscape for Rhodopsin Photoactivation', Journal of Physical Chemistry Letters, vol. 9, no. 24, pp. 7064-7071. https://doi.org/10.1021/acs.jpclett.8b03048
Perera, Suchithranga M.D.C. ; Chawla, Udeep ; Shrestha, Utsab R. ; Bhowmik, Debsindhu ; Struts, Andrey V. ; Qian, Shuo ; Chu, Xiang Qiang ; Brown, Michael F. / Small-Angle Neutron Scattering Reveals Energy Landscape for Rhodopsin Photoactivation. In: Journal of Physical Chemistry Letters. 2018 ; Vol. 9, No. 24. pp. 7064-7071.
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