Small-scale propagation of human iPSCS in serum-free conditions for routine immunocytochemical characterization

Mandi J. Corenblum, Lalitha Madhavan

Research output: Contribution to journalArticle

Abstract

There is great interest in utilizing human induced pluripotent stem cells (hiPSCs) for disease modeling and cell therapeutics due to their patient specificity and characteristic stemness. However, the pluripotency of iPSCs, which is essential to their functionality, must be confirmed before these cells can be used in such applications. While a rigorous characterization of iPSCs, through different cellular and functional assays is necessary to establish their pluripotency, routine assessment of pluripotency maintenance can be achieved more simply and effectively through immunocytochemical techniques. Here, we present a systematic protocol for culturing hiPSCs, in a scaled-down manner, to particularly facilitate the verification of their pluripotent state using immunocytochemistry. More specifically, this methodology encompasses an efficient and cost-effective means of growing iPSCs in serum-free conditions and plating them on small chamber slides or glass coverslips ideal for immunocytochemistry.

Original languageEnglish (US)
Article numbere55260
JournalJournal of Visualized Experiments
Volume2017
Issue number120
DOIs
StatePublished - Feb 18 2017

Keywords

  • Chamber slide
  • Coverslip
  • Developmental biology
  • Feeder-free
  • Immunocytochemistry
  • iPSC
  • Issue 120
  • Pluripotency

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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