Smartphone-based epifluorescence microscope for fresh tissue imaging

Wenbin Zhu, Giacomo Pirovano, Cheng Gong, Nachiket Kulkarni, Christopher David Nguyen, Christian Brand, Thomas Reiner, Dongkyun Kang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a smartphone-based epifluorescence microscope for fresh tissue imaging. The smartphone microscope optics was optimally designed to achieve similar resolution (0.56 µm) and FOV (520 µm) as the bench 40x microscope, commonly used during the histopathologic analysis. Preliminary images obtained from an excised human pancreatic tissue stained with a rapid staining fluorescence dye (PARPi-FL) clearly visualized individual tumor cells.

Original languageEnglish (US)
Title of host publicationNovel Biophotonics Techniques and Applications V
EditorsArjen Amelink, Seemantini K. Nadkarni
PublisherSPIE
ISBN (Electronic)9781510628434
DOIs
StatePublished - Jan 1 2019
EventNovel Biophotonics Techniques and Applications V 2019 - Munich, Germany
Duration: Jun 26 2019Jun 27 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11075
ISSN (Print)1605-7422

Conference

ConferenceNovel Biophotonics Techniques and Applications V 2019
CountryGermany
CityMunich
Period6/26/196/27/19

Fingerprint

Smartphones
Microscopes
microscopes
Tissue
Imaging techniques
Coloring Agents
Fluorescence
Optical resolving power
staining
Staining and Labeling
seats
Tumors
Optics
tumors
Dyes
dyes
Cells
optics
Neoplasms
fluorescence

Keywords

  • fluorescence microscopy
  • fresh tissue imaging
  • point-of-care
  • Smartphone microscope

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Zhu, W., Pirovano, G., Gong, C., Kulkarni, N., Nguyen, C. D., Brand, C., ... Kang, D. (2019). Smartphone-based epifluorescence microscope for fresh tissue imaging. In A. Amelink, & S. K. Nadkarni (Eds.), Novel Biophotonics Techniques and Applications V [110750B] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11075). SPIE. https://doi.org/10.1117/12.2526857

Smartphone-based epifluorescence microscope for fresh tissue imaging. / Zhu, Wenbin; Pirovano, Giacomo; Gong, Cheng; Kulkarni, Nachiket; Nguyen, Christopher David; Brand, Christian; Reiner, Thomas; Kang, Dongkyun.

Novel Biophotonics Techniques and Applications V. ed. / Arjen Amelink; Seemantini K. Nadkarni. SPIE, 2019. 110750B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11075).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhu, W, Pirovano, G, Gong, C, Kulkarni, N, Nguyen, CD, Brand, C, Reiner, T & Kang, D 2019, Smartphone-based epifluorescence microscope for fresh tissue imaging. in A Amelink & SK Nadkarni (eds), Novel Biophotonics Techniques and Applications V., 110750B, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11075, SPIE, Novel Biophotonics Techniques and Applications V 2019, Munich, Germany, 6/26/19. https://doi.org/10.1117/12.2526857
Zhu W, Pirovano G, Gong C, Kulkarni N, Nguyen CD, Brand C et al. Smartphone-based epifluorescence microscope for fresh tissue imaging. In Amelink A, Nadkarni SK, editors, Novel Biophotonics Techniques and Applications V. SPIE. 2019. 110750B. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2526857
Zhu, Wenbin ; Pirovano, Giacomo ; Gong, Cheng ; Kulkarni, Nachiket ; Nguyen, Christopher David ; Brand, Christian ; Reiner, Thomas ; Kang, Dongkyun. / Smartphone-based epifluorescence microscope for fresh tissue imaging. Novel Biophotonics Techniques and Applications V. editor / Arjen Amelink ; Seemantini K. Nadkarni. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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