Smartphone-Based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at the Single Copy Level

Soo Chung, Lane E. Breshears, Sean Perea, Christina M. Morrison, Walter Q. Betancourt, Kelly A Reynolds, Jeong-Yeol Yoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human enteric viruses can be highly infectious and thus capable of causing disease upon ingestion of low doses ranging from 100 to 102 virions. Norovirus is a good example with a minimum infectious dose as low as a few tens of virions, that is, below femtogram scale. Norovirus detection from commonly implicated environmental matrices (water and food) involves complicated concentration of viruses and/or amplification of the norovirus genome, thus rendering detection approaches not feasible for field applications. In this work, norovirus detection was performed on a microfluidic paper analytic device without using any sample concentration or nucleic acid amplification steps by directly imaging and counting on-paper aggregation of antibody-conjugated, fluorescent submicron particles. An in-house developed smartphone-based fluorescence microscope and an image-processing algorithm isolated the particles aggregated by antibody-antigen binding, leading to an extremely low limit of norovirus detection, as low as 1 genome copy/μL in deionized water and 10 genome copies/μL in reclaimed wastewater.

Original languageEnglish (US)
Pages (from-to)11180-11188
Number of pages9
JournalACS Omega
Volume4
Issue number6
DOIs
StatePublished - Jun 27 2019

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Smartphones
Microfluidics
Genes
Viruses
Antibodies
Amplification
Water
Deionized water
Nucleic acids
Antigens
Nucleic Acids
Image processing
Wastewater
Microscopes
Agglomeration
Fluorescence
Imaging techniques

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Smartphone-Based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at the Single Copy Level. / Chung, Soo; Breshears, Lane E.; Perea, Sean; Morrison, Christina M.; Betancourt, Walter Q.; Reynolds, Kelly A; Yoon, Jeong-Yeol.

In: ACS Omega, Vol. 4, No. 6, 27.06.2019, p. 11180-11188.

Research output: Contribution to journalArticle

Chung, Soo ; Breshears, Lane E. ; Perea, Sean ; Morrison, Christina M. ; Betancourt, Walter Q. ; Reynolds, Kelly A ; Yoon, Jeong-Yeol. / Smartphone-Based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at the Single Copy Level. In: ACS Omega. 2019 ; Vol. 4, No. 6. pp. 11180-11188.
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