Smartphone detection of Escherichia coli from wastewater utilizing paper microfluidics

Tu San Park, Dustin K. Harshman, Christopher F. Fronczek, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Smartphone detection of E. coli from wastewater was successfully demonstrated using paper microfluidics. Three channels of each paper chip were pre-loaded with BSA-conjugated and anti-is. coft-conjugated beads with varying amount, for negative control, low- and high-range E. coli detection, respectively. Dipping a paper chip into wastewater (or pipetting) led E. coli antigens to travel through paper fibers while effectively filtering out contaminants. E. coli antigens caused immunoagglutination of antibody-conjugated beads (but not BSA-conjugated beads) that were still confined within the paper fibers. This immunoagglutination was quantified by evaluating Mie scattering from the digital images taken at an optimized angle and distance with a smartphone. The detection limit was single-cell-level and the total assay time was less than one minute.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1347-1349
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Escherichia coli
  • Microfluidics
  • Paper
  • Particle immunoagglutination
  • Smartphone
  • Waste water

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Park, T. S., Harshman, D. K., Fronczek, C. F., & Yoon, J. Y. (2013). Smartphone detection of Escherichia coli from wastewater utilizing paper microfluidics. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1347-1349). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.