Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings

Wenbin Zhu, Giacomo Pirovano, Patrick K. O’Neal, Cheng Gong, Nachiket Kulkarni, Christopher D. Nguyen, Christian Brand, Thomas Reiner, Dongkyun Kang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Disease diagnosis in low-resource settings can be challenging due to the lack of equipment and trained personnel required for histologic analysis. In this paper, we have developed a smartphone-based epifluorescence microscope (SeFM) for imaging fresh tissues at sub-cellular resolution. SeFM provides similar resolution and field of view (FOV) as those used during histologic analysis. The SeFM device achieved the lateral resolution of 0.57 µm and provided microscopy images over a sample area larger than 500 µm. The material cost was low, approximately $3,000. Preliminary images of human pancreatic tumor specimens clearly visualized cellular details. Quantitative analysis showed that using an excess dose of a chemotherapy drug significantly reduced the tumor-specific fluorescence signal, confirming the specificity of the drug and the detection potential of SeFM.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalBiomedical Optics Express
Volume11
Issue number1
DOIs
StatePublished - 2020

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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    Zhu, W., Pirovano, G., O’Neal, P. K., Gong, C., Kulkarni, N., Nguyen, C. D., Brand, C., Reiner, T., & Kang, D. (2020). Smartphone epifluorescence microscopy for cellular imaging of fresh tissue in low-resource settings. Biomedical Optics Express, 11(1), 89-98. https://doi.org/10.1364/BOE.11.000089