Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X

Lisa Y. So, Stephanie J. Munger, Julie Elizabeth Miller

Research output: Contribution to journalArticle

Abstract

Dopamine (DA) is an important neuromodulator of motor control across species. In zebra finches, DA levels vary in song nucleus Area X depending upon social context. DA levels are high and song output is less variable when a male finch sings to a female (female directed, FD) compared to when he is singing by himself (undirected, UD). DA modulates glutamatergic input onto cortico-striatal synapses in Area X via N-methyl-D-aspartate (NMDA) and DA receptor mechanisms, but the relationship to UD vs. FD song output is unclear. Here, we investigate the expression of molecular markers of dopaminergic and glutamatergic synaptic transmission (tyrosine hydroxylase – TH, alpha-synuclein – α-syn) and plasticity (NMDA 2B receptor – GRIN2B) following singing (UD vs. FD) and non-singing states to understand the molecular mechanisms driving differences in song output. We identified relationships between protein levels for these biomarkers in Area X based on singing state and the amount of song, measured as the number of motifs and time spent singing. UD song amount drove increases in TH, α-syn, and NMDA 2B receptor protein levels. By contrast, the amount of FD song did not alter TH and NMDA 2B receptor expression. Levels of α-syn showed differential expression patterns based on UD vs. FD song, consistent with its role in modulating synaptic transmission. We propose a molecular pathway model to explain how social context and amount of song are important drivers of molecular changes required for synaptic transmission and plasticity.

Original languageEnglish (US)
Pages (from-to)103-112
Number of pages10
JournalBehavioural Brain Research
Volume360
DOIs
StatePublished - Mar 15 2019

Fingerprint

Finches
Singing
Music
Basal Ganglia
N-Methyl-D-Aspartate Receptors
Dopamine
Synaptic Transmission
Corpus Striatum
alpha-Synuclein
Neuronal Plasticity
Molecular Models
Equidae
Dopamine Receptors
Tyrosine 3-Monooxygenase
Synapses
Neurotransmitter Agents
Proteins
Biomarkers

Keywords

  • Basal ganglia
  • Dopamine
  • Glutamate
  • Songbird
  • Zebra finch

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Social context-dependent singing alters molecular markers of dopaminergic and glutamatergic signaling in finch basal ganglia Area X. / So, Lisa Y.; Munger, Stephanie J.; Miller, Julie Elizabeth.

In: Behavioural Brain Research, Vol. 360, 15.03.2019, p. 103-112.

Research output: Contribution to journalArticle

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