Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices

J. B. Wijeweera, C. M. Thomas, A Jay Gandolfi, K. Brendel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The present study was undertaken to investigate the usefulness of stress proteins as early, sensitive indicators ofhepa-totoxicity. Induction of stress protein synthesis in precision-cut rat liver slices was examined following in vitro exposure to sodium arsenite or heat shock. Precision-cut rat liver slices were incubated with 10~5 or 10~6 M sodium arsenite for 2, 4 or 8 h in the presence of 3SS-methionine or exposed to hyperthermia (42.5 ± 0.5 °C) for 45 min and then incubated with 35S-methionine for 2, 4 or 8 h. Fluorographic analysis indicated an increase in the synthesis of HSP 70 and HSP 90 family of proteins by both treatments. Immunoblot analysis demonstrated that there was a specific induction of HSP 72 and HSP 90. Induction of HSP 70 was greater than that of HSP 90 by both treatments. Stress protein induction occurred at earlier times by concentrations of arsenite which did not alter other viability parameters such as leakage of intracellular K* or total protein synthesis. The results indicated that induction of stress proteins has the potential usefulness as an early biomarker of arsenite toxicity.

Original languageEnglish (US)
Pages (from-to)35-45
Number of pages11
JournalToxicology
Volume104
Issue number1-3
DOIs
StatePublished - Dec 15 1995

Fingerprint

Heat-Shock Proteins
Liver
Rats
Shock
Hot Temperature
Methionine
Biomarkers
Toxicity
Proteins
Fever
sodium arsenite
arsenite

Keywords

  • HSP 70
  • HSP 90
  • Precision-cut liver slices

ASJC Scopus subject areas

  • Toxicology

Cite this

Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices. / Wijeweera, J. B.; Thomas, C. M.; Gandolfi, A Jay; Brendel, K.

In: Toxicology, Vol. 104, No. 1-3, 15.12.1995, p. 35-45.

Research output: Contribution to journalArticle

Wijeweera, J. B. ; Thomas, C. M. ; Gandolfi, A Jay ; Brendel, K. / Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices. In: Toxicology. 1995 ; Vol. 104, No. 1-3. pp. 35-45.
@article{7a0af39c9e84410ab04c30e06606ccd4,
title = "Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices",
abstract = "The present study was undertaken to investigate the usefulness of stress proteins as early, sensitive indicators ofhepa-totoxicity. Induction of stress protein synthesis in precision-cut rat liver slices was examined following in vitro exposure to sodium arsenite or heat shock. Precision-cut rat liver slices were incubated with 10~5 or 10~6 M sodium arsenite for 2, 4 or 8 h in the presence of 3SS-methionine or exposed to hyperthermia (42.5 ± 0.5 °C) for 45 min and then incubated with 35S-methionine for 2, 4 or 8 h. Fluorographic analysis indicated an increase in the synthesis of HSP 70 and HSP 90 family of proteins by both treatments. Immunoblot analysis demonstrated that there was a specific induction of HSP 72 and HSP 90. Induction of HSP 70 was greater than that of HSP 90 by both treatments. Stress protein induction occurred at earlier times by concentrations of arsenite which did not alter other viability parameters such as leakage of intracellular K* or total protein synthesis. The results indicated that induction of stress proteins has the potential usefulness as an early biomarker of arsenite toxicity.",
keywords = "HSP 70, HSP 90, Precision-cut liver slices",
author = "Wijeweera, {J. B.} and Thomas, {C. M.} and Gandolfi, {A Jay} and K. Brendel",
year = "1995",
month = "12",
day = "15",
doi = "10.1016/0300-483X(95)03119-Z",
language = "English (US)",
volume = "104",
pages = "35--45",
journal = "Toxicology",
issn = "0300-483X",
publisher = "Elsevier Ireland Ltd",
number = "1-3",

}

TY - JOUR

T1 - Sodium arsenite and heat shock induce stress proteins in precision-cut rat liver slices

AU - Wijeweera, J. B.

AU - Thomas, C. M.

AU - Gandolfi, A Jay

AU - Brendel, K.

PY - 1995/12/15

Y1 - 1995/12/15

N2 - The present study was undertaken to investigate the usefulness of stress proteins as early, sensitive indicators ofhepa-totoxicity. Induction of stress protein synthesis in precision-cut rat liver slices was examined following in vitro exposure to sodium arsenite or heat shock. Precision-cut rat liver slices were incubated with 10~5 or 10~6 M sodium arsenite for 2, 4 or 8 h in the presence of 3SS-methionine or exposed to hyperthermia (42.5 ± 0.5 °C) for 45 min and then incubated with 35S-methionine for 2, 4 or 8 h. Fluorographic analysis indicated an increase in the synthesis of HSP 70 and HSP 90 family of proteins by both treatments. Immunoblot analysis demonstrated that there was a specific induction of HSP 72 and HSP 90. Induction of HSP 70 was greater than that of HSP 90 by both treatments. Stress protein induction occurred at earlier times by concentrations of arsenite which did not alter other viability parameters such as leakage of intracellular K* or total protein synthesis. The results indicated that induction of stress proteins has the potential usefulness as an early biomarker of arsenite toxicity.

AB - The present study was undertaken to investigate the usefulness of stress proteins as early, sensitive indicators ofhepa-totoxicity. Induction of stress protein synthesis in precision-cut rat liver slices was examined following in vitro exposure to sodium arsenite or heat shock. Precision-cut rat liver slices were incubated with 10~5 or 10~6 M sodium arsenite for 2, 4 or 8 h in the presence of 3SS-methionine or exposed to hyperthermia (42.5 ± 0.5 °C) for 45 min and then incubated with 35S-methionine for 2, 4 or 8 h. Fluorographic analysis indicated an increase in the synthesis of HSP 70 and HSP 90 family of proteins by both treatments. Immunoblot analysis demonstrated that there was a specific induction of HSP 72 and HSP 90. Induction of HSP 70 was greater than that of HSP 90 by both treatments. Stress protein induction occurred at earlier times by concentrations of arsenite which did not alter other viability parameters such as leakage of intracellular K* or total protein synthesis. The results indicated that induction of stress proteins has the potential usefulness as an early biomarker of arsenite toxicity.

KW - HSP 70

KW - HSP 90

KW - Precision-cut liver slices

UR - http://www.scopus.com/inward/record.url?scp=0029561393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029561393&partnerID=8YFLogxK

U2 - 10.1016/0300-483X(95)03119-Z

DO - 10.1016/0300-483X(95)03119-Z

M3 - Article

C2 - 8560500

AN - SCOPUS:0029561393

VL - 104

SP - 35

EP - 45

JO - Toxicology

JF - Toxicology

SN - 0300-483X

IS - 1-3

ER -