Softening of membrane bilayers by detergents elucidated by deuterium NMR spectroscopy

Dörte Otten, Michael F. Brown, Klaus Beyer

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

Material properties of lipid bilayers were studied on the mesoscopic scale using deuterium nuclear magnetic resonance spectroscopy. The fluid phase of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was compared with DMPC containing a nonionic detergent as an additive. Order parameter profiles were obtained from the deuterium NMR spectra of DMPC having perdeuterated acyl chains (DMPC-J54). A reduction of the order parameters of DMPC-d54 in the presence of the detergent octaethyleneglycol-mono-/i-dodecyl ether (C12E8) was observed, consistent with an increased configurational freedom of the phospholipid acyl chains. Relaxation rates R1z(i) and R1Q(i) were measured and spectral densities Jm(mω0) where in = 1,2 were calculated from the combined relaxation results. Profiles of the observables, i.e., order parameters, relaxation rates, and spectral densities were interpreted within the framework of a new composite membrane deformation model, which describes characteristic properties of the membrane in terms of a continuum picture. According to this model, the influence of the nonionic detergent (C12E8) on the electroneutral DMPC membrane is to increase the membrane flexibility as manifested by the functional dependence of the R1z(i) and R1Q(i) rates, i.e., the dependence of the spectral densities on the corresponding profiles of the orientational order parameters |SCD(i)|. Within the theoretical framework the increased flexibility of the detergent-containing membranes corresponds to a decrease of the elastic constants for continuum (elastic) deformations of the membrane bilayer. In the case of splay fluctuations the elastic constant and the bilayer thickness are related to the macroscopic bending rigidity, which qualitatively yields a correspondence to studies of macroscopic bending fluctuations thus yielding support for the model. In general, these findings indicate a softening of the membrane bilayer by the presence of a nonionic detergent, which corresponds to a decrease of the elastic constants for continuum deformations of the membrane.

Original languageEnglish (US)
Pages (from-to)12119-12129
Number of pages11
JournalJournal of Physical Chemistry B
Volume104
Issue number51
StatePublished - Dec 28 2000

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Softening of membrane bilayers by detergents elucidated by deuterium NMR spectroscopy'. Together they form a unique fingerprint.

  • Cite this