Soil sampling and isolation of Entomopathogenic nematodes (Steinernematidae, Heterorhabditidae)

Rousel A. Orozco, Ming Min Lee, S. Patricia Stock

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Entomopathogenic nematodes (a.k.a. EPN) represent a group of soil-inhabiting nematodes that parasitize a wide range of insects. These nematodes belong to two families: Steinernematidae and Heterorhabditidae. Until now, more than 70 species have been described in the Steinernematidae and there are about 20 species in the Heterorhabditidae. The nematodes have a mutualistic partnership with Enterobacteriaceae bacteria and together they act as a potent insecticidal complex that kills a wide range of insect species. Herein, we focus on the most common techniques considered for collecting EPN from soil. The second part of this presentation focuses on the insect-baiting technique, a widely used approach for the isolation of EPN from soil samples, and the modified White trap technique which is used for the recovery of these nematodes from infected insects. These methods and techniques are key steps for the successful establishment of EPN cultures in the laboratory and also form the basis for other bioassays that consider these nematodes as model organisms for research in other biological disciplines. The techniques shown in this presentation correspond to those performed and/or designed by members of S. P. Stock laboratory as well as those described by various authors.

Original languageEnglish (US)
Article numbere52083
JournalJournal of Visualized Experiments
Issue number89
DOIs
StatePublished - Jul 11 2014

Keywords

  • Entomology
  • Environmental sciences
  • Heterorhabditis
  • Insect-bait
  • Issue 89
  • Modified white-trap
  • Nematodes
  • Nematology
  • Soil sampling
  • Steinernema

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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