Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract

Claude Saliou, Gerald Rimbach, Hadi Moini, Laura McLaughlin, Saeed Hosseini, Jeongmin Lee, Ronald R. Watson, Lester Packer

Research output: Contribution to journalArticle

117 Scopus citations

Abstract

The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-κB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-κB-dependent gene expression in a concentration-dependent manner. However, NF-κB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-κB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-κB-dependent gene expression by PBE possibly contributes to the observed increase in MED.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalFree Radical Biology and Medicine
Volume30
Issue number2
DOIs
StatePublished - Jan 15 2001

Keywords

  • Erythema
  • Free radicals
  • Gene expression
  • Inflammati on
  • Keratinocyte
  • NF-κB
  • Procyanidins
  • Pycnogenol
  • Skin
  • Ultraviolet

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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