Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract

Claude Saliou, Gerald Rimbach, Hadi Moini, Laura McLaughlin, Saeed Hosseini, Jeongmin Lee, Ronald R Watson, Lester Packer

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-κB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-κB-dependent gene expression in a concentration-dependent manner. However, NF-κB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-κB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-κB-dependent gene expression by PBE possibly contributes to the observed increase in MED.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalFree Radical Biology and Medicine
Volume30
Issue number2
DOIs
StatePublished - Jan 15 2001

Fingerprint

Pinus
NF-kappa B
Erythema
Keratinocytes
Gene expression
Skin
Gene Expression
pycnogenols
Ultraviolet Rays
Cell culture
Transcriptional Activation
Oxidation-Reduction
Culture Media
Volunteers
Transcription Factors
Cell Culture Techniques
Chemical activation
Cells
Body Weight
Cell Line

Keywords

  • Erythema
  • Free radicals
  • Gene expression
  • Inflammati on
  • Keratinocyte
  • NF-κB
  • Procyanidins
  • Pycnogenol
  • Skin
  • Ultraviolet

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract. / Saliou, Claude; Rimbach, Gerald; Moini, Hadi; McLaughlin, Laura; Hosseini, Saeed; Lee, Jeongmin; Watson, Ronald R; Packer, Lester.

In: Free Radical Biology and Medicine, Vol. 30, No. 2, 15.01.2001, p. 154-160.

Research output: Contribution to journalArticle

Saliou, Claude ; Rimbach, Gerald ; Moini, Hadi ; McLaughlin, Laura ; Hosseini, Saeed ; Lee, Jeongmin ; Watson, Ronald R ; Packer, Lester. / Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract. In: Free Radical Biology and Medicine. 2001 ; Vol. 30, No. 2. pp. 154-160.
@article{5217b33c88084fb2b84af1a442639661,
title = "Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract",
abstract = "The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-κB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-κB-dependent gene expression in a concentration-dependent manner. However, NF-κB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-κB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-κB-dependent gene expression by PBE possibly contributes to the observed increase in MED.",
keywords = "Erythema, Free radicals, Gene expression, Inflammati on, Keratinocyte, NF-κB, Procyanidins, Pycnogenol, Skin, Ultraviolet",
author = "Claude Saliou and Gerald Rimbach and Hadi Moini and Laura McLaughlin and Saeed Hosseini and Jeongmin Lee and Watson, {Ronald R} and Lester Packer",
year = "2001",
month = "1",
day = "15",
doi = "10.1016/S0891-5849(00)00445-7",
language = "English (US)",
volume = "30",
pages = "154--160",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Solar ultraviolet-induced erythema in human skin and nuclear factor-kappa-B-dependent gene expression in keratinocytes are modulated by a French maritime pine bark extract

AU - Saliou, Claude

AU - Rimbach, Gerald

AU - Moini, Hadi

AU - McLaughlin, Laura

AU - Hosseini, Saeed

AU - Lee, Jeongmin

AU - Watson, Ronald R

AU - Packer, Lester

PY - 2001/1/15

Y1 - 2001/1/15

N2 - The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-κB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-κB-dependent gene expression in a concentration-dependent manner. However, NF-κB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-κB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-κB-dependent gene expression by PBE possibly contributes to the observed increase in MED.

AB - The procyanidin-rich French maritime pine bark extract Pycnogenol (PBE) has been investigated for its effect in protecting human skin against solar UV-simulated light-induced erythema. Twenty-one volunteers were given an oral supplementation of Pycnogenol: 1.10 mg/kg body weight (b. wt.)/d for the first 4 weeks and 1.66 mg/kg b. wt./d for the next 4 weeks. The minimal erythema dose (MED) was measured twice before supplementation (baseline MED), once after the first 4 weeks of supplementation, and a last time at the end of the study. The UVR dose necessary to achieve 1 MED was significantly increased during PBE supplementation. Since the activation of the pro-inflammatory and redox-regulated transcription factor NF-κB is thought to play a major role in UVR-induced erythema, the effect of PBE was also investigated in the human keratinocyte cell line HaCaT. PBE, added to the cell culture medium, inhibited UVR-induced NF-κB-dependent gene expression in a concentration-dependent manner. However, NF-κB-DNA-binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-κB. These data indicate that oral supplementation of PBE reduces erythema in the skin. Inhibition of NF-κB-dependent gene expression by PBE possibly contributes to the observed increase in MED.

KW - Erythema

KW - Free radicals

KW - Gene expression

KW - Inflammati on

KW - Keratinocyte

KW - NF-κB

KW - Procyanidins

KW - Pycnogenol

KW - Skin

KW - Ultraviolet

UR - http://www.scopus.com/inward/record.url?scp=0035863274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035863274&partnerID=8YFLogxK

U2 - 10.1016/S0891-5849(00)00445-7

DO - 10.1016/S0891-5849(00)00445-7

M3 - Article

C2 - 11163532

AN - SCOPUS:0035863274

VL - 30

SP - 154

EP - 160

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 2

ER -