Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats

Ling Fan, Zhao Chen, Wenbin Fu, Nenggui Xu, Jianhua Liu, Aiping Lu, Ziping Li, Shengyong Su, Taixiang Wu, Aihua Ou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague-Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p >. 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.

Original languageEnglish (US)
JournalJAMS Journal of Acupuncture and Meridian Studies
DOIs
StateAccepted/In press - Oct 20 2015

Fingerprint

SNARE Proteins
Type A Botulinum Toxins
Riluzole
Acupuncture
Depression
Vesicle-Associated Membrane Protein 1
Vesicle-Associated Membrane Protein 2
Injections
Depressive Disorder
Reverse Transcription
Sprague Dawley Rats
Sucrose
Glutamic Acid
Hippocampus
Western Blotting

Keywords

  • Acupuncture
  • Antidepressant
  • Botulinum toxin A
  • Riluzole

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Complementary and alternative medicine

Cite this

Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats. / Fan, Ling; Chen, Zhao; Fu, Wenbin; Xu, Nenggui; Liu, Jianhua; Lu, Aiping; Li, Ziping; Su, Shengyong; Wu, Taixiang; Ou, Aihua.

In: JAMS Journal of Acupuncture and Meridian Studies, 20.10.2015.

Research output: Contribution to journalArticle

Fan, Ling ; Chen, Zhao ; Fu, Wenbin ; Xu, Nenggui ; Liu, Jianhua ; Lu, Aiping ; Li, Ziping ; Su, Shengyong ; Wu, Taixiang ; Ou, Aihua. / Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats. In: JAMS Journal of Acupuncture and Meridian Studies. 2015.
@article{ce61094ff0d647f6b92b876aa1599574,
title = "Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats",
abstract = "This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague-Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p >. 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.",
keywords = "Acupuncture, Antidepressant, Botulinum toxin A, Riluzole",
author = "Ling Fan and Zhao Chen and Wenbin Fu and Nenggui Xu and Jianhua Liu and Aiping Lu and Ziping Li and Shengyong Su and Taixiang Wu and Aihua Ou",
year = "2015",
month = "10",
day = "20",
doi = "10.1016/j.jams.2016.04.002",
language = "English (US)",
journal = "JAMS Journal of Acupuncture and Meridian Studies",
issn = "2005-2901",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Soluble N-ethylmaleimide-sensitive Factor Attachment Receptor (SNARE) Protein Involved in the Remission of Depression by Acupuncture in Rats

AU - Fan, Ling

AU - Chen, Zhao

AU - Fu, Wenbin

AU - Xu, Nenggui

AU - Liu, Jianhua

AU - Lu, Aiping

AU - Li, Ziping

AU - Su, Shengyong

AU - Wu, Taixiang

AU - Ou, Aihua

PY - 2015/10/20

Y1 - 2015/10/20

N2 - This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague-Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p >. 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.

AB - This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague-Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture + BTX-A group) or riluzole combined with BTX-A injection (riluzole + BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole + BTX-A and acupuncture + BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole + BTX-A, acupuncture + BTX-A and model groups (p >. 0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole + BTX-A, acupuncture + BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.

KW - Acupuncture

KW - Antidepressant

KW - Botulinum toxin A

KW - Riluzole

UR - http://www.scopus.com/inward/record.url?scp=84973618667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973618667&partnerID=8YFLogxK

U2 - 10.1016/j.jams.2016.04.002

DO - 10.1016/j.jams.2016.04.002

M3 - Article

C2 - 27776762

AN - SCOPUS:84973618667

JO - JAMS Journal of Acupuncture and Meridian Studies

JF - JAMS Journal of Acupuncture and Meridian Studies

SN - 2005-2901

ER -