Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis

W. Craig, A. Wiegand, C. M. O'Neill, R. J. Mathias, J. B. Power, M. R. Davey

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mM 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalPlant Cell Reports
Volume17
Issue number1
DOIs
StatePublished - Nov 1 1997

    Fingerprint

Keywords

  • Brassicaceae
  • C-C intermediate
  • Moricandia arvensis
  • Somatic embryogenesis
  • Stem explants

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Craig, W., Wiegand, A., O'Neill, C. M., Mathias, R. J., Power, J. B., & Davey, M. R. (1997). Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis. Plant Cell Reports, 17(1), 27-31. https://doi.org/10.1007/s002990050346