Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis

Craig A Wissler, A. Wiegand, C. M. O'Neill, R. J. Mathias, J. B. Power, M. R. Davey

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mM 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalPlant Cell Reports
Volume17
Issue number1
DOIs
StatePublished - Nov 1997

Fingerprint

Moricandia arvensis
somatic embryogenesis
explants
stems
Moricandia
insertional mutagenesis
Brassica
somatic embryos
growth regulators
gene transfer
internodes
2,4-D
diploidy
physiology
metabolism
seeds

Keywords

  • Brassicaceae
  • C-C intermediate
  • Moricandia arvensis
  • Somatic embryogenesis
  • Stem explants

ASJC Scopus subject areas

  • Plant Science

Cite this

Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis. / Wissler, Craig A; Wiegand, A.; O'Neill, C. M.; Mathias, R. J.; Power, J. B.; Davey, M. R.

In: Plant Cell Reports, Vol. 17, No. 1, 11.1997, p. 27-31.

Research output: Contribution to journalArticle

Wissler, Craig A ; Wiegand, A. ; O'Neill, C. M. ; Mathias, R. J. ; Power, J. B. ; Davey, M. R. / Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis. In: Plant Cell Reports. 1997 ; Vol. 17, No. 1. pp. 27-31.
@article{29c9debd1807497483769950974856bf,
title = "Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis",
abstract = "Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mM 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90{\%}) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.",
keywords = "Brassicaceae, C-C intermediate, Moricandia arvensis, Somatic embryogenesis, Stem explants",
author = "Wissler, {Craig A} and A. Wiegand and O'Neill, {C. M.} and Mathias, {R. J.} and Power, {J. B.} and Davey, {M. R.}",
year = "1997",
month = "11",
doi = "10.1007/s002990050346",
language = "English (US)",
volume = "17",
pages = "27--31",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis

AU - Wissler, Craig A

AU - Wiegand, A.

AU - O'Neill, C. M.

AU - Mathias, R. J.

AU - Power, J. B.

AU - Davey, M. R.

PY - 1997/11

Y1 - 1997/11

N2 - Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mM 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.

AB - Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mM 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.

KW - Brassicaceae

KW - C-C intermediate

KW - Moricandia arvensis

KW - Somatic embryogenesis

KW - Stem explants

UR - http://www.scopus.com/inward/record.url?scp=0031415994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031415994&partnerID=8YFLogxK

U2 - 10.1007/s002990050346

DO - 10.1007/s002990050346

M3 - Article

AN - SCOPUS:0031415994

VL - 17

SP - 27

EP - 31

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 1

ER -