Somatostatin regulates NHE8 protein expression via the ERK1/2 MAPK pathway in DSS-induced colitis mice

Xiao Li, Lin Cai, Hua Xu, Chong Geng, Jing Lu, Liping Tao, Dan Sun, Fayez K Ghishan, Chunhui Wang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Previous studies reported that administration of somatostatin (SST) to human patients mitigated their diarrheal symptoms. Octreotide (an analog of SST) treatment in animals resulted in upregulation of sodium/hydro-gen exchanger 8 (NHE8). NHE8 is important for water/sodium absorption in the intestine, and loss of NHE8 function results in mucosal injury. Thus we hypothesized that NHE8 expression is inhibited during colitis and that SST treatment during pathological conditions can restore NHE8 expression. Our data showed for the first time that NHE8 is expressed in the human colonic tissue and that NHE8 expression is decreased in ulcerative colitis (UC) patients. We also found that octreotide could stimulate colonic NHE8 expression in colitic mice. Furthermore, the somatostatin receptor 2 (SSTR2) agonist seglitide and the somatostatin receptor 5 (SSTR5) agonist L-817,818 could restore NHE8 expression via its role in suppressing ERK1/2 phosphorylation. Our study uncovered a novel mechanism of SST stimulation of NHE8 expression in colitis.

Original languageEnglish (US)
Pages (from-to)G954-G963
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume311
Issue number5
DOIs
StatePublished - 2016

Fingerprint

MAP Kinase Signaling System
Colitis
Somatostatin
Octreotide
seglitide
Proteins
Sodium
Ulcerative Colitis
Intestines
Up-Regulation
Phosphorylation
Water
Wounds and Injuries
Therapeutics

Keywords

  • Inflammatory bowel disease
  • NHE8
  • Somatostatin
  • SSTR2
  • SSTR5

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Somatostatin regulates NHE8 protein expression via the ERK1/2 MAPK pathway in DSS-induced colitis mice. / Li, Xiao; Cai, Lin; Xu, Hua; Geng, Chong; Lu, Jing; Tao, Liping; Sun, Dan; Ghishan, Fayez K; Wang, Chunhui.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 311, No. 5, 2016, p. G954-G963.

Research output: Contribution to journalArticle

Li, Xiao ; Cai, Lin ; Xu, Hua ; Geng, Chong ; Lu, Jing ; Tao, Liping ; Sun, Dan ; Ghishan, Fayez K ; Wang, Chunhui. / Somatostatin regulates NHE8 protein expression via the ERK1/2 MAPK pathway in DSS-induced colitis mice. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2016 ; Vol. 311, No. 5. pp. G954-G963.
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