Somatostatin regulates tight junction proteins expression in colitis mice

X. Xiao Li, Qian Wang, Hua Xu, Liping Tao, Jing Lu, Lin Cai, Chunhui Wang

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Tight junction plays a critical role in intestinal defence. The alteration and perturbation of tight junction proteins could induce intestine barrier damage, and lead to the malabsorption of electrolytes and water. Previous studies had showed that colonic infection and inflammation could lead to the alteration of tight junction function, and somatostatin could protect intestinal epithelia. Thus, this study could explore that whether somatostatin could regulate tight junction in colitis mice. Colitis mice with diarrhea were induced by Citrobacter rodentium (CR) and Dextran sulfate sodium (DSS). In CR infected model, cladudin-1 and claudin-3 expression significantly decreased compared with the control mice (P < 0.05); after octreotide treatment, claudin-1 and claudin-3 expression significantly increased compared with untreated CR infected mice (P < 0.05). In DSS colitis model, occludin and claudin-3 expression significantly decreased compared with the control mice (P < 0.05); and octreotide treatment could only significantly upregulate claudin-3 expression compared with untreated DSS colitis mice (P < 0.05). To testify our results in vivo, we repeated the models in caco-2 cells by exposed with enteropathogenic Escherichia coli (E. Coli) and Tumor necrosis factor α (TNF-α). The results in vitro were consistent with in vivo study. The results suggested that somatostatin play a role in intestinal barrier protection by modulating tight junction proteins expression.

Original languageEnglish (US)
Pages (from-to)2153-2162
Number of pages10
JournalInternational Journal of Clinical and Experimental Pathology
Volume7
Issue number5
StatePublished - 2014

Keywords

  • Claudins
  • IBD
  • Infectious colitis
  • Occludin
  • Somatostatin
  • Tight junction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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