Spatial gradients of blood vessels and hematopoietic stem and progenitor cells within the marrow cavities of the human skeleton

Vincent A. Bourke, Christopher J Watchman, John D. Reith, Marda L. Jorgensen, Arnaud Dieudonnè, Wesley E. Bolch

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This report evaluates the spatial profile of blood vessel fragments (BVFs) and CD34+ and CD117+ hematopoietic stem and progenitor cells (HSPCs) in human cancellous bone. Bone specimens were sectioned, immunostained (anti-CD34 and anti-CD117), and digitally imaged. Immunoreactive cells and vessels were then optically and morphometrically identified and labeled on the corresponding digital image. The distance of each BVF, or CD34+ or CD117+ HSPC to the nearest trabecular surface was measured and binned in 50-μm increments. The relative concentration of HSPCs and BVFs within cancellous marrow was observed to diminish with increasing distance in the marrow space. On average, 50% of the CD34+ HSPC population, 60% of the CD117+ HSPC population, and 72% of the BVFs were found within 100 μm of the bone surfaces. HSPCs were also found to exist in close proximity to BVFs, which supports the notion of a shared HSPC and vessel spatial niche.

Original languageEnglish (US)
Pages (from-to)4077-4080
Number of pages4
JournalBlood
Volume114
Issue number19
DOIs
StatePublished - Nov 5 2009

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Blood vessels
Hematopoietic Stem Cells
Skeleton
Blood Vessels
Bone Marrow
Bone
Cells
Bone and Bones
Population

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Spatial gradients of blood vessels and hematopoietic stem and progenitor cells within the marrow cavities of the human skeleton. / Bourke, Vincent A.; Watchman, Christopher J; Reith, John D.; Jorgensen, Marda L.; Dieudonnè, Arnaud; Bolch, Wesley E.

In: Blood, Vol. 114, No. 19, 05.11.2009, p. 4077-4080.

Research output: Contribution to journalArticle

Bourke, Vincent A. ; Watchman, Christopher J ; Reith, John D. ; Jorgensen, Marda L. ; Dieudonnè, Arnaud ; Bolch, Wesley E. / Spatial gradients of blood vessels and hematopoietic stem and progenitor cells within the marrow cavities of the human skeleton. In: Blood. 2009 ; Vol. 114, No. 19. pp. 4077-4080.
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