TY - JOUR
T1 - Spatially and temporally regulated α6 integrin cleavage during Xenopus laevis development
AU - Demetriou, Manolis C.
AU - Stylianou, Panayiota
AU - Andreou, Maria
AU - Yiannikouri, Olga
AU - Tsaprailis, George
AU - Cress, Anne E.
AU - Skourides, Paris
N1 - Funding Information:
We thank Dr. Ivan de Curtis (Milan, Italy) for providing us with the α6 integrin antibody. This work was supported by Marie Curie IRGs 036567 and 016613, and the Research Promotion Foundation Grant APONE050502, and CA 56666, CA 23074. Mass spectral proteomic analyses were performed by the Arizona Proteomics Consortium supported by Grants from NIEHS ES06694 and NCI CA023074 and the BIO5 Institute.
PY - 2008/2/15
Y1 - 2008/2/15
N2 - The α6 integrin is essential for early nervous system development in Xenopus laevis. We have previously reported a uPA cleaved form of integrin α6 (α6p), in invasive human prostate cancer tissue, whose presence correlates with increased migration and invasive capacity. We now report that α6 is cleaved during the normal development of Xenopus in a spatially and temporally controlled manner. In addition, unlike normal mammalian tissues, which lack α6p, the major form of the α6 integrin present in adult Xenopus is α6p. The protease responsible for the cleavage in mammals, uPA, is not involved in the cleavage of Xenopus α6. Finally, overexpression of a mammalian α6 mutant which cannot be cleaved leads to developmental abnormalities suggesting a potential role for the cleavage in development.
AB - The α6 integrin is essential for early nervous system development in Xenopus laevis. We have previously reported a uPA cleaved form of integrin α6 (α6p), in invasive human prostate cancer tissue, whose presence correlates with increased migration and invasive capacity. We now report that α6 is cleaved during the normal development of Xenopus in a spatially and temporally controlled manner. In addition, unlike normal mammalian tissues, which lack α6p, the major form of the α6 integrin present in adult Xenopus is α6p. The protease responsible for the cleavage in mammals, uPA, is not involved in the cleavage of Xenopus α6. Finally, overexpression of a mammalian α6 mutant which cannot be cleaved leads to developmental abnormalities suggesting a potential role for the cleavage in development.
KW - Development
KW - Integrin
KW - Xenopus laevis
UR - http://www.scopus.com/inward/record.url?scp=37549061812&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37549061812&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.12.040
DO - 10.1016/j.bbrc.2007.12.040
M3 - Article
C2 - 18083114
AN - SCOPUS:37549061812
VL - 366
SP - 779
EP - 785
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -