Spatially and temporally resolved single-cell exocytosis utilizing individually addressable carbon microelectrode arrays

Bo Zhang, Kelly L. Adams, Sarah J. Luber, Daniel J. Eves, Michael L. Heien, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

121 Scopus citations

Abstract

We report the fabrication and characterization of carbon microelectrode arrays (MEAs) and their application to spatially and temporally resolve neurotransmitter release from single pheochromocytoma (PC12) cells. The carbon MEAs are composed of individually addressable 2.5-μm-radius microdisks embedded in glass. The fabrication involves pulling a multibarrel glass capillary containing a single carbon fiber in each barrel into a sharp tip, followed by beveling the electrode tip to form an array (10-20 μm) of carbon microdisks. This simple fabrication procedure eliminates the need for complicated wiring of the independent electrodes, thus allowing preparation of high-density individually addressable microelectrodes. The carbon MEAs have been characterized using scanning electron microscopy, steady-state and fast-scan voltammetry, and numerical simulations. Amperometric results show that subcellular heterogeneity in single-cell exocytosis can be electrochemically detected with MEAs. These ultrasmall electrochemical probes are suitable for detecting fast chemical events in tight spaces, as well as for developing multifunctional electrochemical microsensors.

Original languageEnglish (US)
Pages (from-to)1394-1400
Number of pages7
JournalAnalytical chemistry
Volume80
Issue number5
DOIs
StatePublished - Mar 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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