Spatiotemporal dynamics of microRNA during epithelial collective cell migration

Zachary S. Dean, Reza Riahi, Pak Kin Wong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs variably involved in a wide variety of developmental and regenerative programs. Techniques for monitoring the spatiotemporal expression of miRNA in living cells are essential to elucidate the roles of miRNA during these complex regulatory processes. The small size, low abundance, sequence similarity, and degradation susceptibility of miRNAs, however, make their detection challenging. In this study, we detail a double-stranded locked nucleic acid (dsLNA) probe for detecting intracellular miRNAs during epithelial collective migration. The dsLNA probe is capable of detecting the dynamic regulation and dose-dependent modulation of miRNAs. The probe is applied to monitor the spatial distribution of miRNA expression of a migrating epithelium. Our results reveal a gradient of miRNA over the first one hundred microns from the leading edge and show the involvement of miR-21 in the complex regulation of transforming growth factor beta modulated epithelial migration. With its ease of use and capacity for real-time monitoring of miRNAs in living cells, the dsLNA probe carries the potential for studying the function and regulation of miRNA in a wide spectrum of complex biological processes.

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
JournalBiomaterials
Volume37
DOIs
StatePublished - Jan 1 2015

Fingerprint

MicroRNAs
Cell Movement
Nucleic acids
Epithelial Cells
Nucleic Acid Probes
Cells
Monitoring
RNA
Spatial distribution
Modulation
Degradation
Biological Phenomena
Small Untranslated RNA
Transforming Growth Factor beta
Epithelium

Keywords

  • Collective migration
  • Intracellular detection
  • MicroRNA
  • Single cell
  • TGF-β

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Spatiotemporal dynamics of microRNA during epithelial collective cell migration. / Dean, Zachary S.; Riahi, Reza; Wong, Pak Kin.

In: Biomaterials, Vol. 37, 01.01.2015, p. 156-163.

Research output: Contribution to journalArticle

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