Specific binding of cancer cells using a microchamber array functionalized with antibodies

Xiangjun Zheng, Siu Lun Cheung, Lian Wang, Joyce Schroeder, Ronald L Heimark, James C Baygents, Roberto Z Guzman, Yitshak Zohar

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Specific binding of target suspended metastatic cancer cells to an antibody-functionalized surface utilizing a microfluidic device has experimentally been investigated under various conditions. The microfluidic devices, fabricated in silicon using DRIE process, consisted of a 5×5 micochamber array; each 1mm×1mm in area, and 50pm in depth. The oxide surface of the microchammber array was functionalized with various antibodies immobilized on a protein G layer. The microfluidic device design allows accurate counting of cells loaded into each microchamber and, thus, enabling a reliable counting of cells captured from homogeneous suspensions. The effects of cell suspension concentration, incubation times and ambient temperature on cell capture efficiency have been examined. Furthermore, to evaluate the specificity of the cell-surface interaction, several cell cancer types expressing different membrane receptors have been incubated on surfaces functionalized with various counter receptors. Specific binding of up to 100% of the suspended cells is observed when using surfaces functionalized with counter receptors matching the cell receptors; otherwise, non-specific binding of less than 15% of suspended cells is obtained if the functionalized counter receptors do not match the cell receptors.

Original languageEnglish (US)
Title of host publicationASME International Mechanical Engineering Congress and Exposition, Proceedings
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages821-827
Number of pages7
Volume12
EditionPART B
ISBN (Print)9780791843857
DOIs
StatePublished - 2010
EventASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009 - Lake Buena Vista, FL, United States
Duration: Nov 13 2009Nov 19 2009

Other

OtherASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009
CountryUnited States
CityLake Buena Vista, FL
Period11/13/0911/19/09

Fingerprint

Antibodies
Cells
Microfluidics
Proteins
Membranes
Silicon
Oxides
Temperature

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Zheng, X., Cheung, S. L., Wang, L., Schroeder, J., Heimark, R. L., Baygents, J. C., ... Zohar, Y. (2010). Specific binding of cancer cells using a microchamber array functionalized with antibodies. In ASME International Mechanical Engineering Congress and Exposition, Proceedings (PART B ed., Vol. 12, pp. 821-827). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2009-13217

Specific binding of cancer cells using a microchamber array functionalized with antibodies. / Zheng, Xiangjun; Cheung, Siu Lun; Wang, Lian; Schroeder, Joyce; Heimark, Ronald L; Baygents, James C; Guzman, Roberto Z; Zohar, Yitshak.

ASME International Mechanical Engineering Congress and Exposition, Proceedings. Vol. 12 PART B. ed. American Society of Mechanical Engineers (ASME), 2010. p. 821-827.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zheng, X, Cheung, SL, Wang, L, Schroeder, J, Heimark, RL, Baygents, JC, Guzman, RZ & Zohar, Y 2010, Specific binding of cancer cells using a microchamber array functionalized with antibodies. in ASME International Mechanical Engineering Congress and Exposition, Proceedings. PART B edn, vol. 12, American Society of Mechanical Engineers (ASME), pp. 821-827, ASME 2009 International Mechanical Engineering Congress and Exposition, IMECE2009, Lake Buena Vista, FL, United States, 11/13/09. https://doi.org/10.1115/IMECE2009-13217
Zheng X, Cheung SL, Wang L, Schroeder J, Heimark RL, Baygents JC et al. Specific binding of cancer cells using a microchamber array functionalized with antibodies. In ASME International Mechanical Engineering Congress and Exposition, Proceedings. PART B ed. Vol. 12. American Society of Mechanical Engineers (ASME). 2010. p. 821-827 https://doi.org/10.1115/IMECE2009-13217
Zheng, Xiangjun ; Cheung, Siu Lun ; Wang, Lian ; Schroeder, Joyce ; Heimark, Ronald L ; Baygents, James C ; Guzman, Roberto Z ; Zohar, Yitshak. / Specific binding of cancer cells using a microchamber array functionalized with antibodies. ASME International Mechanical Engineering Congress and Exposition, Proceedings. Vol. 12 PART B. ed. American Society of Mechanical Engineers (ASME), 2010. pp. 821-827
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