Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting

Elena V. Rosca, Jill M. Stukel, Robert J. Gillies, Josef Vagner, Michael R. Caplan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Effective targeting of drugs to cells requires that the drug reach the target cell and interact specifically with it. In this study, we synthesized a biomacromolecular, multivalent construct intended to target glioblastoma tumors. The construct was created by linking three dodecapeptides, reported to bind the α6β 1 integrin, with poly(ethylene glycol) linkers. The construct is intended to be delivered locally, and it demonstrates a more homogeneous and more rapid perfusion profile in comparison with quantum dots. The binding specificity of the construct was investigated by using glioblastoma cells and normal human astrocyte cells. The results reveal qualitative differences in binding between glioma and normal human astrocyte cells, with a moderate increase in binding avidity due to multivalency (0.79 μM for the trivalent construct versus 4.28 μM for the dodecapeptide). Overall, biomacromolecular constructs appear to be a promising approach for targeting with high biocompatibility, good perfusion abilities, and specificity.

Original languageEnglish (US)
Pages (from-to)3830-3835
Number of pages6
JournalBiomacromolecules
Volume8
Issue number12
DOIs
StatePublished - Dec 2007

Fingerprint

Biocompatibility
Integrins
Pharmaceutical Preparations
Polyethylene glycols
Semiconductor quantum dots
Tumors
Glioblastoma
Astrocytes
Perfusion
Quantum Dots
Ethylene Glycol
Drug Delivery Systems
Glioma
Neoplasms

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting. / Rosca, Elena V.; Stukel, Jill M.; Gillies, Robert J.; Vagner, Josef; Caplan, Michael R.

In: Biomacromolecules, Vol. 8, No. 12, 12.2007, p. 3830-3835.

Research output: Contribution to journalArticle

Rosca, Elena V. ; Stukel, Jill M. ; Gillies, Robert J. ; Vagner, Josef ; Caplan, Michael R. / Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting. In: Biomacromolecules. 2007 ; Vol. 8, No. 12. pp. 3830-3835.
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