Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta

Hong Lei, Carolina E. Reisenman, Caroline H. Wilson, Prasad Gabbur, John G Hildebrand

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bursting as well as tonic firing patterns have been described in various sensory systems. In the olfactory system, spontaneous bursts have been observed in neurons distributed across several synaptic levels, from the periphery, to the olfactory bulb (OB) and to the olfactory cortex. Several in vitro studies indicate that spontaneous firing patterns may be viewed as "fingerprints" of different types of neurons that exhibit distinct functions in the OB. It is still not known, however, if and how neuronal burstiness is correlated with the coding of natural olfactory stimuli. We thus conducted an in vivo study to probe this question in the OB equivalent structure of insects, the antennal lobe (AL) of the tobacco hornworm Manduca sexta. We found that in the moth's AL, both projection (output) neurons (PNs) and local interneurons (LNs) are spontaneously active, but PNs tend to produce spike bursts while LNs fire more regularly. In addition, we found that the burstiness of PNs is correlated with the strength of their responses to odor stimulation - the more bursting the stronger their responses to odors. Moreover, the burstiness of PNs was also positively correlated with the spontaneous firing rate of these neurons, and pharmacological reduction of bursting resulted in a decrease of the neurons' responsiveness. These results suggest that neuronal burstiness reflects a physiological state of these neurons that is directly linked to their response characteristics.

Original languageEnglish (US)
Article numbere23382
JournalPLoS One
Volume6
Issue number8
DOIs
StatePublished - Aug 29 2011

Fingerprint

antennal lobe
Manduca
Tobacco
Manduca sexta
Neurons
neurons
olfactory bulb
Olfactory Bulb
interneurons
Odors
Interneurons
odors
sensory system
Moths
Dermatoglyphics
in vivo studies
physiological state
in vitro studies
moths
Insects

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta. / Lei, Hong; Reisenman, Carolina E.; Wilson, Caroline H.; Gabbur, Prasad; Hildebrand, John G.

In: PLoS One, Vol. 6, No. 8, e23382, 29.08.2011.

Research output: Contribution to journalArticle

Lei, Hong ; Reisenman, Carolina E. ; Wilson, Caroline H. ; Gabbur, Prasad ; Hildebrand, John G. / Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta. In: PLoS One. 2011 ; Vol. 6, No. 8.
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