Splanchnic and mammary nitrogen metabolism by dairy cows fed dry-rolled or steam-flaked sorghum grain

A. Delgado-Elorduy, C. B. Theurer, J. T. Huber, A. Alio, O. Lozano, M. Sadik, P. Cuneo, H. D. De Young, I. J. Simas, J. E.P. Santos, L. Nussio, C. Nussio, K. E. Webb, H. Tagari

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19 Scopus citations

Abstract

Objectives: were to determine net release or uptake of α-amino N, ammonia N, and urea N across portal-drained viscera, liver, splanchnic, and mammary tissues of lactating Holstein cows (n = 8, 86 ± 8 d in milk) fed alfalfa hay-based total mixed rations containing 40% dry-rolled or steam-flaked sorghum grain. The total mixed rations were offered at 12-h intervals in a crossover design. Blood samples were obtained from indwelling catheters in the portal, hepatic, and mammary veins and mesenteric or costoabdominal arteries, every 2 h for each cow and diet. Steam-flaking increased in vitro rate of starch hydrolysis compared with dry-rolled sorghum (66 vs. 25%). Diet did not alter dry matter intake (18.2 ± 0.3 kg). Daily milk yield (27.6 ± 0.8 kg), efficiency of production, and most milk components did not differ between diets, but fat yield was reduced (0.86 vs. 0.91 kg/d) by steam-flaked sorghum, and lactose concentration was increased (4.99 vs. 4.82%). Blood flows in portal and hepatic veins did not differ between diets. Steam-flaking tended to increase urea N cycling to the gut (162 vs. 95 g/d) compared with dry-rolling of sorghum, whereas net absorption of ammonia N and α-amino N across portal-drained viscera were decreased. Net mammary uptake of α-amino N increased more than 20% (83 vs. 67 g/d), resulting in a higher mammary extraction ratio (15 vs. 11%) for steam-flaked versus dry-rolled sorghum. Flaking of sorghum improved the efficiency of postabsorptive N metabolism by increasing urea N cycled to the gut and α-amino N uptake by the mammary gland.

Original languageEnglish (US)
Pages (from-to)148-159
Number of pages12
JournalJournal of dairy science
Volume85
Issue number1
DOIs
StatePublished - Jan 2002

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Keywords

  • Dairy cows
  • Flaked sorghum
  • Nitrogen metabolism

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Delgado-Elorduy, A., Theurer, C. B., Huber, J. T., Alio, A., Lozano, O., Sadik, M., Cuneo, P., De Young, H. D., Simas, I. J., Santos, J. E. P., Nussio, L., Nussio, C., Webb, K. E., & Tagari, H. (2002). Splanchnic and mammary nitrogen metabolism by dairy cows fed dry-rolled or steam-flaked sorghum grain. Journal of dairy science, 85(1), 148-159. https://doi.org/10.3168/jds.S0022-0302(02)74063-0