Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities

A. Alio, C. B. Theurer, O. Lozano, J. T. Huber, R. S. Swingle, A. Delgado-Elorduy, S Peder Cuneo, D. DeYoung, K. E. Webb

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The objective of this study was to determine effects of processing method, dry-rolled (DR) vs steam-flaked (SF), and degree of processing (flake density, FD) of SF sorghum grain on splanchnic (gut and liver) N metabolism by growing steers. Diets contained 77% sorghum grain either DR or SF at densities of 437, 360, and 283 g/L (SF34, SF28, and SF22, respectively). Eight crossbred steers (340 kg initial BW), implanted with indwelling catheters into portal, hepatic, and mesenteric veins and the mesenteric artery, were used in a randomized complete block design. Blood flows and net output or uptake of ammonia N, urea N (UN), and α-amino N (AAN) were measured across portal-drained viscera, hepatic, and splanchnic tissues. Plasma arterial, portal, and hepatic concentrations of individual amino acids were also measured. Decreasing FD linearly increased (P = .04) net absorption of AAN (51, 73, and 78 g/d for SF34, SF28, and SF22, respectively) and transfer (cycling) of blood UN to the gut (49, 48 and 64 g/d; P = .02). Net UN cycling averaged 38% of N intake across all diets. Hepatic uptake of AAN or UN synthesis, and splanchnic output of AAN and UN, were not altered by FD. Lowering FD linearly increased (P ≤ .02) portal-arterial concentration differences for blood AAN and UN and plasma arterial concentrations for alanine. Steers fed SF compared to DR tended to have greater (P = .11) blood UN cycling (percentage of hepatic synthesis; 64 vs 50%) and decreased (P = .03) net splanchnic UN output (30 vs 50 g/d), but other net fluxes of N were not altered across splanchnic tissues. Steam-flaking compared to dry-rolling tended to decrease (P = .12) portal, but not hepatic, blood flow and increased (P < .01) hepatic-arterial concentration differences for blood UN. Except for a decrease (P = .01) in hepatic-arterial concentration differences of glutamine, plasma amino acid concentrations were not altered by feeding SF vs DR sorghum. Processing method (steam-flaking vs dry-rolling) or increasing the degree of processing (by decreasing FD) of SF sorghum grain resulted in greater transfer of blood UN to the gut. Reducing FD also linearly increased the absorption of AAN by growing steers, which explains (in part) published responses of superior performance by steers fed SF grains.

Original languageEnglish (US)
Pages (from-to)1355-1363
Number of pages9
JournalJournal of Animal Science
Volume78
Issue number5
StatePublished - May 2000

Fingerprint

Sorghum
Viscera
grain sorghum
nitrogen metabolism
Steam
beef cattle
Urea
steam
Nitrogen
urea
flakes
Diet
liver
diet
Liver
splanchnic tissues
blood
flaking
digestive system
processing technology

Keywords

  • Beef steers
  • Flaking
  • Nitrogen Metabolism

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Alio, A., Theurer, C. B., Lozano, O., Huber, J. T., Swingle, R. S., Delgado-Elorduy, A., ... Webb, K. E. (2000). Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities. Journal of Animal Science, 78(5), 1355-1363.

Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities. / Alio, A.; Theurer, C. B.; Lozano, O.; Huber, J. T.; Swingle, R. S.; Delgado-Elorduy, A.; Cuneo, S Peder; DeYoung, D.; Webb, K. E.

In: Journal of Animal Science, Vol. 78, No. 5, 05.2000, p. 1355-1363.

Research output: Contribution to journalArticle

Alio, A, Theurer, CB, Lozano, O, Huber, JT, Swingle, RS, Delgado-Elorduy, A, Cuneo, SP, DeYoung, D & Webb, KE 2000, 'Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities', Journal of Animal Science, vol. 78, no. 5, pp. 1355-1363.
Alio A, Theurer CB, Lozano O, Huber JT, Swingle RS, Delgado-Elorduy A et al. Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities. Journal of Animal Science. 2000 May;78(5):1355-1363.
Alio, A. ; Theurer, C. B. ; Lozano, O. ; Huber, J. T. ; Swingle, R. S. ; Delgado-Elorduy, A. ; Cuneo, S Peder ; DeYoung, D. ; Webb, K. E. / Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities. In: Journal of Animal Science. 2000 ; Vol. 78, No. 5. pp. 1355-1363.
@article{bb1b71dff054449dbd167da5483dd132,
title = "Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities",
abstract = "The objective of this study was to determine effects of processing method, dry-rolled (DR) vs steam-flaked (SF), and degree of processing (flake density, FD) of SF sorghum grain on splanchnic (gut and liver) N metabolism by growing steers. Diets contained 77{\%} sorghum grain either DR or SF at densities of 437, 360, and 283 g/L (SF34, SF28, and SF22, respectively). Eight crossbred steers (340 kg initial BW), implanted with indwelling catheters into portal, hepatic, and mesenteric veins and the mesenteric artery, were used in a randomized complete block design. Blood flows and net output or uptake of ammonia N, urea N (UN), and α-amino N (AAN) were measured across portal-drained viscera, hepatic, and splanchnic tissues. Plasma arterial, portal, and hepatic concentrations of individual amino acids were also measured. Decreasing FD linearly increased (P = .04) net absorption of AAN (51, 73, and 78 g/d for SF34, SF28, and SF22, respectively) and transfer (cycling) of blood UN to the gut (49, 48 and 64 g/d; P = .02). Net UN cycling averaged 38{\%} of N intake across all diets. Hepatic uptake of AAN or UN synthesis, and splanchnic output of AAN and UN, were not altered by FD. Lowering FD linearly increased (P ≤ .02) portal-arterial concentration differences for blood AAN and UN and plasma arterial concentrations for alanine. Steers fed SF compared to DR tended to have greater (P = .11) blood UN cycling (percentage of hepatic synthesis; 64 vs 50{\%}) and decreased (P = .03) net splanchnic UN output (30 vs 50 g/d), but other net fluxes of N were not altered across splanchnic tissues. Steam-flaking compared to dry-rolling tended to decrease (P = .12) portal, but not hepatic, blood flow and increased (P < .01) hepatic-arterial concentration differences for blood UN. Except for a decrease (P = .01) in hepatic-arterial concentration differences of glutamine, plasma amino acid concentrations were not altered by feeding SF vs DR sorghum. Processing method (steam-flaking vs dry-rolling) or increasing the degree of processing (by decreasing FD) of SF sorghum grain resulted in greater transfer of blood UN to the gut. Reducing FD also linearly increased the absorption of AAN by growing steers, which explains (in part) published responses of superior performance by steers fed SF grains.",
keywords = "Beef steers, Flaking, Nitrogen Metabolism",
author = "A. Alio and Theurer, {C. B.} and O. Lozano and Huber, {J. T.} and Swingle, {R. S.} and A. Delgado-Elorduy and Cuneo, {S Peder} and D. DeYoung and Webb, {K. E.}",
year = "2000",
month = "5",
language = "English (US)",
volume = "78",
pages = "1355--1363",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "5",

}

TY - JOUR

T1 - Splanchnic nitrogen metabolism by growing beef steers fed diets containing sorghum grain flaked at different densities

AU - Alio, A.

AU - Theurer, C. B.

AU - Lozano, O.

AU - Huber, J. T.

AU - Swingle, R. S.

AU - Delgado-Elorduy, A.

AU - Cuneo, S Peder

AU - DeYoung, D.

AU - Webb, K. E.

PY - 2000/5

Y1 - 2000/5

N2 - The objective of this study was to determine effects of processing method, dry-rolled (DR) vs steam-flaked (SF), and degree of processing (flake density, FD) of SF sorghum grain on splanchnic (gut and liver) N metabolism by growing steers. Diets contained 77% sorghum grain either DR or SF at densities of 437, 360, and 283 g/L (SF34, SF28, and SF22, respectively). Eight crossbred steers (340 kg initial BW), implanted with indwelling catheters into portal, hepatic, and mesenteric veins and the mesenteric artery, were used in a randomized complete block design. Blood flows and net output or uptake of ammonia N, urea N (UN), and α-amino N (AAN) were measured across portal-drained viscera, hepatic, and splanchnic tissues. Plasma arterial, portal, and hepatic concentrations of individual amino acids were also measured. Decreasing FD linearly increased (P = .04) net absorption of AAN (51, 73, and 78 g/d for SF34, SF28, and SF22, respectively) and transfer (cycling) of blood UN to the gut (49, 48 and 64 g/d; P = .02). Net UN cycling averaged 38% of N intake across all diets. Hepatic uptake of AAN or UN synthesis, and splanchnic output of AAN and UN, were not altered by FD. Lowering FD linearly increased (P ≤ .02) portal-arterial concentration differences for blood AAN and UN and plasma arterial concentrations for alanine. Steers fed SF compared to DR tended to have greater (P = .11) blood UN cycling (percentage of hepatic synthesis; 64 vs 50%) and decreased (P = .03) net splanchnic UN output (30 vs 50 g/d), but other net fluxes of N were not altered across splanchnic tissues. Steam-flaking compared to dry-rolling tended to decrease (P = .12) portal, but not hepatic, blood flow and increased (P < .01) hepatic-arterial concentration differences for blood UN. Except for a decrease (P = .01) in hepatic-arterial concentration differences of glutamine, plasma amino acid concentrations were not altered by feeding SF vs DR sorghum. Processing method (steam-flaking vs dry-rolling) or increasing the degree of processing (by decreasing FD) of SF sorghum grain resulted in greater transfer of blood UN to the gut. Reducing FD also linearly increased the absorption of AAN by growing steers, which explains (in part) published responses of superior performance by steers fed SF grains.

AB - The objective of this study was to determine effects of processing method, dry-rolled (DR) vs steam-flaked (SF), and degree of processing (flake density, FD) of SF sorghum grain on splanchnic (gut and liver) N metabolism by growing steers. Diets contained 77% sorghum grain either DR or SF at densities of 437, 360, and 283 g/L (SF34, SF28, and SF22, respectively). Eight crossbred steers (340 kg initial BW), implanted with indwelling catheters into portal, hepatic, and mesenteric veins and the mesenteric artery, were used in a randomized complete block design. Blood flows and net output or uptake of ammonia N, urea N (UN), and α-amino N (AAN) were measured across portal-drained viscera, hepatic, and splanchnic tissues. Plasma arterial, portal, and hepatic concentrations of individual amino acids were also measured. Decreasing FD linearly increased (P = .04) net absorption of AAN (51, 73, and 78 g/d for SF34, SF28, and SF22, respectively) and transfer (cycling) of blood UN to the gut (49, 48 and 64 g/d; P = .02). Net UN cycling averaged 38% of N intake across all diets. Hepatic uptake of AAN or UN synthesis, and splanchnic output of AAN and UN, were not altered by FD. Lowering FD linearly increased (P ≤ .02) portal-arterial concentration differences for blood AAN and UN and plasma arterial concentrations for alanine. Steers fed SF compared to DR tended to have greater (P = .11) blood UN cycling (percentage of hepatic synthesis; 64 vs 50%) and decreased (P = .03) net splanchnic UN output (30 vs 50 g/d), but other net fluxes of N were not altered across splanchnic tissues. Steam-flaking compared to dry-rolling tended to decrease (P = .12) portal, but not hepatic, blood flow and increased (P < .01) hepatic-arterial concentration differences for blood UN. Except for a decrease (P = .01) in hepatic-arterial concentration differences of glutamine, plasma amino acid concentrations were not altered by feeding SF vs DR sorghum. Processing method (steam-flaking vs dry-rolling) or increasing the degree of processing (by decreasing FD) of SF sorghum grain resulted in greater transfer of blood UN to the gut. Reducing FD also linearly increased the absorption of AAN by growing steers, which explains (in part) published responses of superior performance by steers fed SF grains.

KW - Beef steers

KW - Flaking

KW - Nitrogen Metabolism

UR - http://www.scopus.com/inward/record.url?scp=0034181751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034181751&partnerID=8YFLogxK

M3 - Article

C2 - 10834592

AN - SCOPUS:0034181751

VL - 78

SP - 1355

EP - 1363

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 5

ER -