Stability studies of tetracycline in methanol solution

Yuanling Liang, M Bonner Denton, Robert B. Bates

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The stability of tetracycline in methanol solution was investigated by UV-visible spectroscopy, HPLC and TLC methods. After dissolution in methanol, tetracycline decomposed rapidly under the influence of light and atmospheric oxygen, forming more than fourteen different degradation products. None of the previously reported degradation products, such as the epi- and anhydro-compounds, were detected as the final degradation products. The molecular structures for eight of the compounds were suggested by their product-ion mass spectra. A degradation sequence was proposed for the reactions of tetracycline with methanol. A new HPLC-MS mobile phase was developed, which solved the clogging problem at the interface between the HPLC and MS chamber and enabled a high separation efficiency. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)45-55
Number of pages11
JournalJournal of Chromatography A
Volume827
Issue number1
DOIs
StatePublished - Dec 4 1998

Fingerprint

Tetracycline
Methanol
High Pressure Liquid Chromatography
Degradation
Molecular Structure
Spectrum Analysis
Ions
Molecular structure
Oxygen
Byproducts
Light
Dissolution
Spectroscopy

Keywords

  • Antibiotics
  • Mass spectrometry
  • Methanol
  • Stability studies
  • Tetracyclines

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Stability studies of tetracycline in methanol solution. / Liang, Yuanling; Denton, M Bonner; Bates, Robert B.

In: Journal of Chromatography A, Vol. 827, No. 1, 04.12.1998, p. 45-55.

Research output: Contribution to journalArticle

Liang, Yuanling ; Denton, M Bonner ; Bates, Robert B. / Stability studies of tetracycline in methanol solution. In: Journal of Chromatography A. 1998 ; Vol. 827, No. 1. pp. 45-55.
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