Stabilized phospholipid membranes in chromatography: Toward membrane protein-functionalized stationary phases

Elyssia S. Gallagher, Elisabeth Mansfield, Craig A Aspinwall

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Transmembrane protein (TMP)-functionalized materials have resulted in powerful new methods in chemical analysis. Of particular interest is the development of high-throughput, TMP-functionalized stationary phases for affinity chromatography of complex mixtures of analytes. Several natural and synthetic phospholipids and lipid mimics have been used for TMP reconstitution, although the resulting membranes often lack the requisite chemical and temporal stability for long-term use, a problem that is exacerbated in flowing separation systems. Polymerizable lipids with markedly increased membrane stability and TMP functionality have been developed over the past two decades. More recently, these lipids have been incorporated into a range of analytical methods, including separation techniques, and are now poised to have a significant impact on TMP-based separations. Here, we describe current methods for preparing TMP-containing stationary phases and examine the potential utility of polymerizable lipids in TMP affinity chromatography.

Original languageEnglish (US)
Pages (from-to)2223-2229
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number9-10
DOIs
Publication statusPublished - 2014

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Keywords

  • Affinity chromatography
  • Immobilized artificial membrane
  • Immobilized liposome chromatography
  • Polymerizable phospholipid
  • Stationary phase
  • Transmembrane protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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