Stable formation of compositionally unique stress granules in virus-infected cells

Joanna Piotrowska, Spencer J. Hansen, Nogi Park, Katarzyna Jamka, Peter Sarnow, Kurt E Gustin

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Stress granules are sites of mRNA storage formed in response to a variety of stresses, including viral infections. Here, the mechanisms and consequences of stress granule formation during poliovirus infection were examined. The results indicate that stress granules containing T-cell-restricted intracellular antigen 1 (TIA-1) and mRNA are stably constituted in infected cells despite lacking intact RasGAP SH3-domain binding protein 1 (G3BP) and eukaryotic initiation factor 4G. Fluorescent in situ hybridization revealed that stress granules in infected cells do not contain significant amounts of viral positive-strand RNA. Infection does not prevent stress granule formation in response to heat shock, indicating that poliovirus does not block de novo stress granule formation. A mutant TIA-1 protein that prevents stress granule formation during oxidative stress also prevents formation in infected cells. However, stress granule formation during infection is more dependent upon ongoing transcription than is formation during oxidative stress or heat shock. Furthermore, Sam68 is recruited to stress granules in infected cells but not to stress granules formed in response to oxidative stress or heat shock. These results demonstrate that stress granule formation in poliovirus-infected cells utilizes a transcription-dependent pathway that results in the appearance of stable, compositionally unique stress granules.

Original languageEnglish (US)
Pages (from-to)3654-3665
Number of pages12
JournalJournal of Virology
Volume84
Issue number7
DOIs
StatePublished - Apr 2010

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granules
Poliovirus
Viruses
viruses
Oxidative Stress
cells
Eukaryotic Initiation Factor-4G
Shock
Enterovirus C
Hot Temperature
Infection
T-Lymphocytes
Antigens
Heat-Shock Response
Messenger RNA
src Homology Domains
heat stress
Virus Diseases
Heat-Shock Proteins
Fluorescence In Situ Hybridization

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Stable formation of compositionally unique stress granules in virus-infected cells. / Piotrowska, Joanna; Hansen, Spencer J.; Park, Nogi; Jamka, Katarzyna; Sarnow, Peter; Gustin, Kurt E.

In: Journal of Virology, Vol. 84, No. 7, 04.2010, p. 3654-3665.

Research output: Contribution to journalArticle

Piotrowska, Joanna ; Hansen, Spencer J. ; Park, Nogi ; Jamka, Katarzyna ; Sarnow, Peter ; Gustin, Kurt E. / Stable formation of compositionally unique stress granules in virus-infected cells. In: Journal of Virology. 2010 ; Vol. 84, No. 7. pp. 3654-3665.
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