Standardized transportation of human islets: An islet cell resource center study of more than 2,000 shipments

John S. Kaddis, Matthew S. Hanson, James Cravens, Dajun Qian, Barbara Olack, Martha Antler, Klearchos K Papas, Itzia Iglesias, Barbara Barbaro, Luis Fernandez, Alvin C. Powers, Joyce C. Niland

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Preservation of cell quality during shipment of human pancreatic islets for use in laboratory research is a crucial, but neglected, topic. Mammalian cells, including islets, have been shown to be adversely affected by temperature changes in vitro and in vivo, yet protocols that control for thermal fluctuations during cell transport are lacking. To evaluate an optimal method of shipping human islets, an initial assessment of transportation conditions was conducted using standardized materials and operating procedures in 48 shipments sent to a central location by eight pancreas-processing laboratories using a single commercial airline transporter. Optimization of preliminary conditions was conducted, and human islet quality was then evaluated in 2,338 shipments pre- and postimplementation of a finalized transportation container and standard operating procedures. The initial assessment revealed that the outside temperature ranged from a mean of -4.6 ± 10.3°C to 20.9 ± 4.8°C. Within-container temperature drops to or below 15°C occurred in 16 shipments (36%), while the temperature was found to be stabilized between 15°C and 29°C in 29 shipments (64%). Implementation of an optimized transportation container and operating procedure reduced the number of within-container temperature drops (£15°C) to 13% (n = 37 of 289 winter shipments), improved the number desirably maintained between 15°C and 29°C to 86% (n = 250), but also increased the number reaching or exceeding 29°C to 1% (n = 2; overall p < 0.0001). Additionally, postreceipt quality ratings of excellent to good improved pre- versus postimplantation of the standardized protocol, adjusting for preshipment purity/viability levels (p < 0.0001). Our results show that extreme temperature fluctuations during transport of human islets, occurring when using a commercial airline transporter for long distance shipping, can be controlled using standardized containers, materials, and operating procedures. This cost-effective and pragmatic standardized protocol for the transportation of human islets can potentially be adapted for use with other mammalian cell systems and is available online at http://iidp.coh.org/sops.aspx.

Original languageEnglish (US)
Pages (from-to)1101-1111
Number of pages11
JournalCell Transplantation
Volume22
Issue number7
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Islets of Langerhans
Containers
Temperature
Freight transportation
Cells
Research laboratories
Pancreas
Hot Temperature
Costs and Cost Analysis
Processing
Research
Costs

Keywords

  • Diabetes
  • Human islets
  • Insulin
  • Shipping protocol
  • Temperature control

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Kaddis, J. S., Hanson, M. S., Cravens, J., Qian, D., Olack, B., Antler, M., ... Niland, J. C. (2013). Standardized transportation of human islets: An islet cell resource center study of more than 2,000 shipments. Cell Transplantation, 22(7), 1101-1111. https://doi.org/10.3727/096368912X653219

Standardized transportation of human islets : An islet cell resource center study of more than 2,000 shipments. / Kaddis, John S.; Hanson, Matthew S.; Cravens, James; Qian, Dajun; Olack, Barbara; Antler, Martha; Papas, Klearchos K; Iglesias, Itzia; Barbaro, Barbara; Fernandez, Luis; Powers, Alvin C.; Niland, Joyce C.

In: Cell Transplantation, Vol. 22, No. 7, 2013, p. 1101-1111.

Research output: Contribution to journalArticle

Kaddis, JS, Hanson, MS, Cravens, J, Qian, D, Olack, B, Antler, M, Papas, KK, Iglesias, I, Barbaro, B, Fernandez, L, Powers, AC & Niland, JC 2013, 'Standardized transportation of human islets: An islet cell resource center study of more than 2,000 shipments', Cell Transplantation, vol. 22, no. 7, pp. 1101-1111. https://doi.org/10.3727/096368912X653219
Kaddis, John S. ; Hanson, Matthew S. ; Cravens, James ; Qian, Dajun ; Olack, Barbara ; Antler, Martha ; Papas, Klearchos K ; Iglesias, Itzia ; Barbaro, Barbara ; Fernandez, Luis ; Powers, Alvin C. ; Niland, Joyce C. / Standardized transportation of human islets : An islet cell resource center study of more than 2,000 shipments. In: Cell Transplantation. 2013 ; Vol. 22, No. 7. pp. 1101-1111.
@article{ed88a63e6d414cca93089ead2083329d,
title = "Standardized transportation of human islets: An islet cell resource center study of more than 2,000 shipments",
abstract = "Preservation of cell quality during shipment of human pancreatic islets for use in laboratory research is a crucial, but neglected, topic. Mammalian cells, including islets, have been shown to be adversely affected by temperature changes in vitro and in vivo, yet protocols that control for thermal fluctuations during cell transport are lacking. To evaluate an optimal method of shipping human islets, an initial assessment of transportation conditions was conducted using standardized materials and operating procedures in 48 shipments sent to a central location by eight pancreas-processing laboratories using a single commercial airline transporter. Optimization of preliminary conditions was conducted, and human islet quality was then evaluated in 2,338 shipments pre- and postimplementation of a finalized transportation container and standard operating procedures. The initial assessment revealed that the outside temperature ranged from a mean of -4.6 ± 10.3°C to 20.9 ± 4.8°C. Within-container temperature drops to or below 15°C occurred in 16 shipments (36{\%}), while the temperature was found to be stabilized between 15°C and 29°C in 29 shipments (64{\%}). Implementation of an optimized transportation container and operating procedure reduced the number of within-container temperature drops (£15°C) to 13{\%} (n = 37 of 289 winter shipments), improved the number desirably maintained between 15°C and 29°C to 86{\%} (n = 250), but also increased the number reaching or exceeding 29°C to 1{\%} (n = 2; overall p < 0.0001). Additionally, postreceipt quality ratings of excellent to good improved pre- versus postimplantation of the standardized protocol, adjusting for preshipment purity/viability levels (p < 0.0001). Our results show that extreme temperature fluctuations during transport of human islets, occurring when using a commercial airline transporter for long distance shipping, can be controlled using standardized containers, materials, and operating procedures. This cost-effective and pragmatic standardized protocol for the transportation of human islets can potentially be adapted for use with other mammalian cell systems and is available online at http://iidp.coh.org/sops.aspx.",
keywords = "Diabetes, Human islets, Insulin, Shipping protocol, Temperature control",
author = "Kaddis, {John S.} and Hanson, {Matthew S.} and James Cravens and Dajun Qian and Barbara Olack and Martha Antler and Papas, {Klearchos K} and Itzia Iglesias and Barbara Barbaro and Luis Fernandez and Powers, {Alvin C.} and Niland, {Joyce C.}",
year = "2013",
doi = "10.3727/096368912X653219",
language = "English (US)",
volume = "22",
pages = "1101--1111",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "7",

}

TY - JOUR

T1 - Standardized transportation of human islets

T2 - An islet cell resource center study of more than 2,000 shipments

AU - Kaddis, John S.

AU - Hanson, Matthew S.

AU - Cravens, James

AU - Qian, Dajun

AU - Olack, Barbara

AU - Antler, Martha

AU - Papas, Klearchos K

AU - Iglesias, Itzia

AU - Barbaro, Barbara

AU - Fernandez, Luis

AU - Powers, Alvin C.

AU - Niland, Joyce C.

PY - 2013

Y1 - 2013

N2 - Preservation of cell quality during shipment of human pancreatic islets for use in laboratory research is a crucial, but neglected, topic. Mammalian cells, including islets, have been shown to be adversely affected by temperature changes in vitro and in vivo, yet protocols that control for thermal fluctuations during cell transport are lacking. To evaluate an optimal method of shipping human islets, an initial assessment of transportation conditions was conducted using standardized materials and operating procedures in 48 shipments sent to a central location by eight pancreas-processing laboratories using a single commercial airline transporter. Optimization of preliminary conditions was conducted, and human islet quality was then evaluated in 2,338 shipments pre- and postimplementation of a finalized transportation container and standard operating procedures. The initial assessment revealed that the outside temperature ranged from a mean of -4.6 ± 10.3°C to 20.9 ± 4.8°C. Within-container temperature drops to or below 15°C occurred in 16 shipments (36%), while the temperature was found to be stabilized between 15°C and 29°C in 29 shipments (64%). Implementation of an optimized transportation container and operating procedure reduced the number of within-container temperature drops (£15°C) to 13% (n = 37 of 289 winter shipments), improved the number desirably maintained between 15°C and 29°C to 86% (n = 250), but also increased the number reaching or exceeding 29°C to 1% (n = 2; overall p < 0.0001). Additionally, postreceipt quality ratings of excellent to good improved pre- versus postimplantation of the standardized protocol, adjusting for preshipment purity/viability levels (p < 0.0001). Our results show that extreme temperature fluctuations during transport of human islets, occurring when using a commercial airline transporter for long distance shipping, can be controlled using standardized containers, materials, and operating procedures. This cost-effective and pragmatic standardized protocol for the transportation of human islets can potentially be adapted for use with other mammalian cell systems and is available online at http://iidp.coh.org/sops.aspx.

AB - Preservation of cell quality during shipment of human pancreatic islets for use in laboratory research is a crucial, but neglected, topic. Mammalian cells, including islets, have been shown to be adversely affected by temperature changes in vitro and in vivo, yet protocols that control for thermal fluctuations during cell transport are lacking. To evaluate an optimal method of shipping human islets, an initial assessment of transportation conditions was conducted using standardized materials and operating procedures in 48 shipments sent to a central location by eight pancreas-processing laboratories using a single commercial airline transporter. Optimization of preliminary conditions was conducted, and human islet quality was then evaluated in 2,338 shipments pre- and postimplementation of a finalized transportation container and standard operating procedures. The initial assessment revealed that the outside temperature ranged from a mean of -4.6 ± 10.3°C to 20.9 ± 4.8°C. Within-container temperature drops to or below 15°C occurred in 16 shipments (36%), while the temperature was found to be stabilized between 15°C and 29°C in 29 shipments (64%). Implementation of an optimized transportation container and operating procedure reduced the number of within-container temperature drops (£15°C) to 13% (n = 37 of 289 winter shipments), improved the number desirably maintained between 15°C and 29°C to 86% (n = 250), but also increased the number reaching or exceeding 29°C to 1% (n = 2; overall p < 0.0001). Additionally, postreceipt quality ratings of excellent to good improved pre- versus postimplantation of the standardized protocol, adjusting for preshipment purity/viability levels (p < 0.0001). Our results show that extreme temperature fluctuations during transport of human islets, occurring when using a commercial airline transporter for long distance shipping, can be controlled using standardized containers, materials, and operating procedures. This cost-effective and pragmatic standardized protocol for the transportation of human islets can potentially be adapted for use with other mammalian cell systems and is available online at http://iidp.coh.org/sops.aspx.

KW - Diabetes

KW - Human islets

KW - Insulin

KW - Shipping protocol

KW - Temperature control

UR - http://www.scopus.com/inward/record.url?scp=84879589470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879589470&partnerID=8YFLogxK

U2 - 10.3727/096368912X653219

DO - 10.3727/096368912X653219

M3 - Article

C2 - 22889479

AN - SCOPUS:84879589470

VL - 22

SP - 1101

EP - 1111

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 7

ER -