Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes

Arup Chakraborty, Heddwen L Brooks, Ping Zhang, Wayne Smith, Matthew R. McReynolds, Jay B. Hoying, Roger Bick, Luan Truong, Brian Poindexter, Hui Lan, Wafa Elbjeirami, David Sheikh-Hamad

Research output: Contribution to journalArticle

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Abstract

The mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) inhibits monocyte chemotactic protein-1- and stromal-derived factor-1α (SDF-1α)-mediated chemotaxis and diminishes chemokinesis in macrophage-like RAW264.7 and U937 cells in a manner that may involve attenuation of the intracellular calcium signal. STC1 is strongly induced in the kidney following obstructive injury. We hypothesized that STC1 may serve to attenuate the influx of inflammatory cells to the site of tissue injury. In this study, we examined the effect of STC1 on the migration of freshly isolated human macrophages, neutrophils, and T and B lymphocytes through quiescent or IL-1β-treated human umbilical vein endothelial cell (HUVEC) monolayers. STC1 inhibited transmigration of macrophages and T lymphocytes through quiescent or IL-1β-activated HUVECs but did not attenuate the transmigration of neutrophils and B lymphocytes. STC1 regulates gene expression in cultured endothelial cells and is detected on the apical surface of endothelial cells in vivo. The data suggest that STC1 plays a critical role in transendothelial migration of inflammatory cells and is involved in the regulation of numerous aspects of endothelial function.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume292
Issue number2
DOIs
StatePublished - Feb 2007

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Transendothelial and Transepithelial Migration
Leukocytes
Gene Expression
Macrophages
Interleukin-1
Neutrophils
B-Lymphocytes
Endothelial Cells
Calcium
T-Lymphocytes
U937 Cells
Chemokine CCL2
Human Umbilical Vein Endothelial Cells
Wounds and Injuries
Chemotaxis
teleocalcin
Cultured Cells
Fishes
Hormones
Kidney

Keywords

  • Array
  • Calcium regulation
  • Inflammation
  • Vascular biology

ASJC Scopus subject areas

  • Physiology

Cite this

Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes. / Chakraborty, Arup; Brooks, Heddwen L; Zhang, Ping; Smith, Wayne; McReynolds, Matthew R.; Hoying, Jay B.; Bick, Roger; Truong, Luan; Poindexter, Brian; Lan, Hui; Elbjeirami, Wafa; Sheikh-Hamad, David.

In: American Journal of Physiology - Renal Physiology, Vol. 292, No. 2, 02.2007.

Research output: Contribution to journalArticle

Chakraborty, A, Brooks, HL, Zhang, P, Smith, W, McReynolds, MR, Hoying, JB, Bick, R, Truong, L, Poindexter, B, Lan, H, Elbjeirami, W & Sheikh-Hamad, D 2007, 'Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes', American Journal of Physiology - Renal Physiology, vol. 292, no. 2. https://doi.org/10.1152/ajprenal.00219.2006
Chakraborty, Arup ; Brooks, Heddwen L ; Zhang, Ping ; Smith, Wayne ; McReynolds, Matthew R. ; Hoying, Jay B. ; Bick, Roger ; Truong, Luan ; Poindexter, Brian ; Lan, Hui ; Elbjeirami, Wafa ; Sheikh-Hamad, David. / Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes. In: American Journal of Physiology - Renal Physiology. 2007 ; Vol. 292, No. 2.
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