Statistical modeling and analyses of a base-specific Salmonella mutagenicity assay

Walter W. Piegorsch, Susan J. Simmons, Barry H. Margolin, Errol Zeiger, Xavier M. Gidrol, Pauline Gee

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Statistical features of a base-specific Salmonella mutagenicity assay are considered in detail, following up on a previous report comparing responses of base-specific Salmonella (Ames II(TM)) strains with those of traditional tester strains. In addition to using different Salmonella strains, the new procedure also differs in that it is performed as a microwell fluctuation test, as opposed to the standard plate or preincubation test. This report describes the statistical modeling of data obtained from the use of these new strains in the microwell test procedure. We emphasize how to assess any significant interactions between replicate cultures and exposure doses, and how to identify a significant increase in the mutagenic response to a series of concentrations of a test substance. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume467
Issue number1
DOIs
StatePublished - Apr 13 2000
Externally publishedYes

Keywords

  • Ames II strains
  • Complementary log-log link function
  • Culture-by-dose interaction
  • Fluctuation test
  • Generalized linear model
  • His mutant tester strains
  • Many-to-one testing
  • Multiple comparisons with a control
  • Salmonella typhimurium
  • Statistical methods

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

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