Status of pink bollworm susceptibility to Bt in Arizona

A. L. Patin, T. J. Dennehy, M. A. Sims, Bruce E Tabashnik, Y. B. Liu, L. Antilla, Dawn H Gouge, T. J. Henneberry, R. Staten

Research output: Chapter in Book/Report/Conference proceedingConference contribution

30 Citations (Scopus)

Abstract

Statewide monitoring of pink bollworm susceptibility to Bt toxin was conducted in Arizona in 1997 and 1998. In bioassays in which Bt toxin was incorporated into insect diet, a concentration of 10 μg/ml Cry 1 Ac caused >80% mortality of all field populations. LC50s of Arizona populations ranged from 0.35 to 1.7 μg Cry 1 Ac/ml of insect diet. A laboratory strain of pink bollworm had an LC50 of 0.53 μg/ml. A selection experiment was conducted in which survivors of bioassays of 3.2 and 10 μg/ml of Cry 1 Ac from all Arizona populations tested in 1997 were pooled and reared for one generation on diet containing 10 μg Cry 1 Ac/ml. Selection produced a strain of pink bollworm that was 100- to 460-fold less susceptible to Cry 1 Ac, than were the field populations from which it was derived. Preliminary results from greenhouse evaluations concerning survival of this resistant strain on Bt cotton showed that larvae were able to complete development in bolls of Bt cotton, pupate, and successfully reproduce. We conclude from these preliminary results that the type of resistance we have isolated in the laboratory is likely to negatively impact field performance of Bt cotton in Arizona in the future. However, we found no evidence that field performance of Bt cotton has yet been compromised. In large field trials, in-field refuges of non-Bt cotton were contrasted with the standard, external refuges. During both 1997 and 1998, in-field refuge plots yielded comparable to, or better than, plots of 100% Bt cotton, yet they produced substantial numbers of pink bollworm late in the season on non-Bt plants.

Original languageEnglish (US)
Title of host publicationProceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA
EditorsP. Dugger, D. Richter, P. Dugger, D. Richter
PublisherNational Cotton Council of America
Pages991-996
Number of pages6
StatePublished - 1999
EventProceedings of the 1999 Beltwide Cotton Conference - Orlando, United States
Duration: Jan 3 1999Jan 7 1999

Other

OtherProceedings of the 1999 Beltwide Cotton Conference
CountryUnited States
CityOrlando
Period1/3/991/7/99

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Cotton
Nutrition
Bioassay
Greenhouses
Monitoring
Experiments

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Patin, A. L., Dennehy, T. J., Sims, M. A., Tabashnik, B. E., Liu, Y. B., Antilla, L., ... Staten, R. (1999). Status of pink bollworm susceptibility to Bt in Arizona. In P. Dugger, D. Richter, P. Dugger, & D. Richter (Eds.), Proceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA (pp. 991-996). National Cotton Council of America.

Status of pink bollworm susceptibility to Bt in Arizona. / Patin, A. L.; Dennehy, T. J.; Sims, M. A.; Tabashnik, Bruce E; Liu, Y. B.; Antilla, L.; Gouge, Dawn H; Henneberry, T. J.; Staten, R.

Proceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA. ed. / P. Dugger; D. Richter; P. Dugger; D. Richter. National Cotton Council of America, 1999. p. 991-996.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Patin, AL, Dennehy, TJ, Sims, MA, Tabashnik, BE, Liu, YB, Antilla, L, Gouge, DH, Henneberry, TJ & Staten, R 1999, Status of pink bollworm susceptibility to Bt in Arizona. in P Dugger, D Richter, P Dugger & D Richter (eds), Proceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA. National Cotton Council of America, pp. 991-996, Proceedings of the 1999 Beltwide Cotton Conference, Orlando, United States, 1/3/99.
Patin AL, Dennehy TJ, Sims MA, Tabashnik BE, Liu YB, Antilla L et al. Status of pink bollworm susceptibility to Bt in Arizona. In Dugger P, Richter D, Dugger P, Richter D, editors, Proceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA. National Cotton Council of America. 1999. p. 991-996
Patin, A. L. ; Dennehy, T. J. ; Sims, M. A. ; Tabashnik, Bruce E ; Liu, Y. B. ; Antilla, L. ; Gouge, Dawn H ; Henneberry, T. J. ; Staten, R. / Status of pink bollworm susceptibility to Bt in Arizona. Proceedings of the 1999 Beltwide Cotton Conference, January 1999, Orlando, Florida, USA. editor / P. Dugger ; D. Richter ; P. Dugger ; D. Richter. National Cotton Council of America, 1999. pp. 991-996
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abstract = "Statewide monitoring of pink bollworm susceptibility to Bt toxin was conducted in Arizona in 1997 and 1998. In bioassays in which Bt toxin was incorporated into insect diet, a concentration of 10 μg/ml Cry 1 Ac caused >80{\%} mortality of all field populations. LC50s of Arizona populations ranged from 0.35 to 1.7 μg Cry 1 Ac/ml of insect diet. A laboratory strain of pink bollworm had an LC50 of 0.53 μg/ml. A selection experiment was conducted in which survivors of bioassays of 3.2 and 10 μg/ml of Cry 1 Ac from all Arizona populations tested in 1997 were pooled and reared for one generation on diet containing 10 μg Cry 1 Ac/ml. Selection produced a strain of pink bollworm that was 100- to 460-fold less susceptible to Cry 1 Ac, than were the field populations from which it was derived. Preliminary results from greenhouse evaluations concerning survival of this resistant strain on Bt cotton showed that larvae were able to complete development in bolls of Bt cotton, pupate, and successfully reproduce. We conclude from these preliminary results that the type of resistance we have isolated in the laboratory is likely to negatively impact field performance of Bt cotton in Arizona in the future. However, we found no evidence that field performance of Bt cotton has yet been compromised. In large field trials, in-field refuges of non-Bt cotton were contrasted with the standard, external refuges. During both 1997 and 1998, in-field refuge plots yielded comparable to, or better than, plots of 100{\%} Bt cotton, yet they produced substantial numbers of pink bollworm late in the season on non-Bt plants.",
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