Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies

Scott A. Barbee, Patricia S. Estes, Anne Marie Cziko, Jens Hillebrand, Rene A. Luedeman, Jeff M. Coller, Nick Johnson, Iris C. Howlett, Cuiyun Geng, Ryu Ueda, Andrea H. Brand, Sarah F. Newbury, James E. Wilhelm, Richard B Levine, Akira Nakamura, Roy Parker, Mani Ramaswami

Research output: Contribution to journalArticle

251 Citations (Scopus)

Abstract

Local control of mRNA translation modulates neuronal development, synaptic plasticity, and memory formation. A poorly understood aspect of this control is the role and composition of ribonucleoprotein (RNP) particles that mediate transport and translation of neuronal RNAs. Here, we show that staufen- and FMRP-containing RNPs in Drosophila neurons contain proteins also present in somatic "P bodies," including the RNA-degradative enzymes Dcp1p and Xrn1p/Pacman and crucial components of miRNA (argonaute), NMD (Upf1p), and general translational repression (Dhh1p/Me31B) pathways. Drosophila Me31B is shown to participate (1) with an FMRP-associated, P body protein (Scd6p/trailer hitch) in FMRP-driven, argonaute-dependent translational repression in developing eye imaginal discs; (2) in dendritic elaboration of larval sensory neurons; and (3) in bantam miRNA-mediated translational repression in wing imaginal discs. These results argue for a conserved mechanism of translational control critical to neuronal function and open up new experimental avenues for understanding the regulation of mRNA function within neurons.

Original languageEnglish (US)
Pages (from-to)997-1009
Number of pages13
JournalNeuron
Volume52
Issue number6
DOIs
StatePublished - Dec 21 2006

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Imaginal Discs
MicroRNAs
Drosophila
RNA
Neurons
Neuronal Plasticity
Ribonucleoproteins
Protein Biosynthesis
Sensory Receptor Cells
Proteins
Messenger RNA
Enzymes

Keywords

  • MOLNEURO
  • RNA
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Barbee, S. A., Estes, P. S., Cziko, A. M., Hillebrand, J., Luedeman, R. A., Coller, J. M., ... Ramaswami, M. (2006). Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies. Neuron, 52(6), 997-1009. https://doi.org/10.1016/j.neuron.2006.10.028

Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies. / Barbee, Scott A.; Estes, Patricia S.; Cziko, Anne Marie; Hillebrand, Jens; Luedeman, Rene A.; Coller, Jeff M.; Johnson, Nick; Howlett, Iris C.; Geng, Cuiyun; Ueda, Ryu; Brand, Andrea H.; Newbury, Sarah F.; Wilhelm, James E.; Levine, Richard B; Nakamura, Akira; Parker, Roy; Ramaswami, Mani.

In: Neuron, Vol. 52, No. 6, 21.12.2006, p. 997-1009.

Research output: Contribution to journalArticle

Barbee, SA, Estes, PS, Cziko, AM, Hillebrand, J, Luedeman, RA, Coller, JM, Johnson, N, Howlett, IC, Geng, C, Ueda, R, Brand, AH, Newbury, SF, Wilhelm, JE, Levine, RB, Nakamura, A, Parker, R & Ramaswami, M 2006, 'Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies', Neuron, vol. 52, no. 6, pp. 997-1009. https://doi.org/10.1016/j.neuron.2006.10.028
Barbee SA, Estes PS, Cziko AM, Hillebrand J, Luedeman RA, Coller JM et al. Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies. Neuron. 2006 Dec 21;52(6):997-1009. https://doi.org/10.1016/j.neuron.2006.10.028
Barbee, Scott A. ; Estes, Patricia S. ; Cziko, Anne Marie ; Hillebrand, Jens ; Luedeman, Rene A. ; Coller, Jeff M. ; Johnson, Nick ; Howlett, Iris C. ; Geng, Cuiyun ; Ueda, Ryu ; Brand, Andrea H. ; Newbury, Sarah F. ; Wilhelm, James E. ; Levine, Richard B ; Nakamura, Akira ; Parker, Roy ; Ramaswami, Mani. / Staufen- and FMRP-Containing Neuronal RNPs Are Structurally and Functionally Related to Somatic P Bodies. In: Neuron. 2006 ; Vol. 52, No. 6. pp. 997-1009.
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