Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model

Kelly L. Adams, Johan Engelbrektsson, Marina Voinova, Bo Zhang, Daniel J. Eves, Roger Karlsson, Michael L Heien, Ann Sofie Cans, Andrew G. Ewing

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters △i (change in current) and nanotube length. Catechol was used to determine the △i attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.

Original languageEnglish (US)
Pages (from-to)1020-1026
Number of pages7
JournalAnalytical Chemistry
Volume82
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

Fingerprint

Nanotubes
Fick's laws
Fluxes
Membranes
Lipids
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Adams, K. L., Engelbrektsson, J., Voinova, M., Zhang, B., Eves, D. J., Karlsson, R., ... Ewing, A. G. (2010). Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model. Analytical Chemistry, 82(3), 1020-1026. https://doi.org/10.1021/ac902282d

Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model. / Adams, Kelly L.; Engelbrektsson, Johan; Voinova, Marina; Zhang, Bo; Eves, Daniel J.; Karlsson, Roger; Heien, Michael L; Cans, Ann Sofie; Ewing, Andrew G.

In: Analytical Chemistry, Vol. 82, No. 3, 01.02.2010, p. 1020-1026.

Research output: Contribution to journalArticle

Adams, KL, Engelbrektsson, J, Voinova, M, Zhang, B, Eves, DJ, Karlsson, R, Heien, ML, Cans, AS & Ewing, AG 2010, 'Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model', Analytical Chemistry, vol. 82, no. 3, pp. 1020-1026. https://doi.org/10.1021/ac902282d
Adams KL, Engelbrektsson J, Voinova M, Zhang B, Eves DJ, Karlsson R et al. Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model. Analytical Chemistry. 2010 Feb 1;82(3):1020-1026. https://doi.org/10.1021/ac902282d
Adams, Kelly L. ; Engelbrektsson, Johan ; Voinova, Marina ; Zhang, Bo ; Eves, Daniel J. ; Karlsson, Roger ; Heien, Michael L ; Cans, Ann Sofie ; Ewing, Andrew G. / Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model. In: Analytical Chemistry. 2010 ; Vol. 82, No. 3. pp. 1020-1026.
@article{d1c91bac61124002a5d1e3723354f4e0,
title = "Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model",
abstract = "By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters △i (change in current) and nanotube length. Catechol was used to determine the △i attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.",
author = "Adams, {Kelly L.} and Johan Engelbrektsson and Marina Voinova and Bo Zhang and Eves, {Daniel J.} and Roger Karlsson and Heien, {Michael L} and Cans, {Ann Sofie} and Ewing, {Andrew G.}",
year = "2010",
month = "2",
day = "1",
doi = "10.1021/ac902282d",
language = "English (US)",
volume = "82",
pages = "1020--1026",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model

AU - Adams, Kelly L.

AU - Engelbrektsson, Johan

AU - Voinova, Marina

AU - Zhang, Bo

AU - Eves, Daniel J.

AU - Karlsson, Roger

AU - Heien, Michael L

AU - Cans, Ann Sofie

AU - Ewing, Andrew G.

PY - 2010/2/1

Y1 - 2010/2/1

N2 - By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters △i (change in current) and nanotube length. Catechol was used to determine the △i attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.

AB - By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters △i (change in current) and nanotube length. Catechol was used to determine the △i attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.

UR - http://www.scopus.com/inward/record.url?scp=76149140480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76149140480&partnerID=8YFLogxK

U2 - 10.1021/ac902282d

DO - 10.1021/ac902282d

M3 - Article

C2 - 20039639

AN - SCOPUS:76149140480

VL - 82

SP - 1020

EP - 1026

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 3

ER -