Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model

Kelly L. Adams, Johan Engelbrektsson, Marina Voinova, Bo Zhang, Daniel J. Eves, Roger Karlsson, Michael L. Heien, Ann Sofie Cans, Andrew G. Ewing

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick's first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick's law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters △i (change in current) and nanotube length. Catechol was used to determine the △i attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.

Original languageEnglish (US)
Pages (from-to)1020-1026
Number of pages7
JournalAnalytical chemistry
Volume82
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model'. Together they form a unique fingerprint.

  • Cite this

    Adams, K. L., Engelbrektsson, J., Voinova, M., Zhang, B., Eves, D. J., Karlsson, R., Heien, M. L., Cans, A. S., & Ewing, A. G. (2010). Steady-state electrochemical determination of lipidie nanotube diameter utilizing an artificial cell model. Analytical chemistry, 82(3), 1020-1026. https://doi.org/10.1021/ac902282d