Steroid Hormone Receptor-mediated Histone Deacetylation and Transcription at the Mouse Mammary Tumor Virus Promoter

Lynn A. Sheldon, Matthias Becker, Catharine Smith

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Acetylation of lysines in histones H3 and H4 N-terminal tails is associated with transcriptional activation and deacetylation with repression. Our studies with the mouse mammary tumor virus (MMTV) promoter in chromatin show significant levels of acetylation at promoter proximal and distal regions prior to transactivation. Upon activation with glucocorticoids or progestins, promoter proximal histones become deacetylated within the region of inducible nuclease hypersensitivity. The deacetylation lags behind the initiation of transcription, indicating a role in post-activation regulation. Our results indicate a novel mechanism by which target promoters are regulated by steroid receptors and chromatin modification machinery.

Original languageEnglish (US)
Pages (from-to)32423-32426
Number of pages4
JournalJournal of Biological Chemistry
Volume276
Issue number35
DOIs
StatePublished - Aug 31 2001
Externally publishedYes

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Steroid hormones
Mouse mammary tumor virus
Steroid Receptors
Transcription
Viruses
Carcinogens
Histones
Tumors
Acetylation
Chemical activation
Hormones
Transcriptional Activation
Chromatin
Progestins
Glucocorticoids
Lysine
Machinery
Hypersensitivity

ASJC Scopus subject areas

  • Biochemistry

Cite this

Steroid Hormone Receptor-mediated Histone Deacetylation and Transcription at the Mouse Mammary Tumor Virus Promoter. / Sheldon, Lynn A.; Becker, Matthias; Smith, Catharine.

In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32423-32426.

Research output: Contribution to journalArticle

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