Steroidogenic capacity of residual ovarian tissue in 4-vinylcyclohexene diepoxide- treated mice

Zelieann Rivera, Patricia J. Christian, Sam L. Marion, Heddwen L Brooks, Patricia B Hoyer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Menopause is an important public health issue because of its association with a number of disorders. Androgens produced by residual ovarian tissue after menopause could impact the development of these disorders. It has been unclear, however, whether the postmenopausal ovary retains steroidogenic capacity. Thus, an ovary-intact mouse model for menopause that uses the occupational chemical 4-vinylcyclohexene diepoxide (VCD) was used to characterize the expression of steroidogenic genes in residual ovarian tissue of follicle-depleted mice. Female B6C3F1 mice (age, 28 days) were dosed daily for 20 days with either vehicle or VCD (160 mg kg -1 day -1) to induce ovarian failure. Ovaries were collected on Day 181 and analyzed for mRNA and protein. Acyclic aged mice were used as controls for natural ovarian senescence. Relative to cycling controls, expression of mRNA encoding steroidogenic acute regulatory protein (Star); cholesterol side-chain cleavage (Cyp11a1); 3beta-hydroxysteroid dehydrogenase (Hsd3b); 17alpha-hydrox- ylase (Cyp17a1); scavenger receptor class B, type 1 (Scarbl); low-density lipoprotein receptor (Ldlr); and luteinizing hormone receptor (Lhcgr) was enriched in VCD-treated ovaries. In acyclic aged ovaries, mRNA expression for only Cyp17a1 and Lhcgr was greater than that in controls. Compared to cycling controls, ovaries from VCD-treated and aged mice had similar levels of HSD3B, CYP17A1, and LHCGR protein. The pattern of protein immunofluorescence staining for HSD3B in follicle- depleted (VCD-treated) ovaries was homogeneous, whereas that for CYP17A1 was only seen in residual interstitial cells. Circulating levels of FSH and LH were increased, and androstenedione levels were detectable following follicle depletion in VCD-treated mice. These findings support the idea that residual ovarian tissue in VCD-treated mice retains androgenic capacity.

Original languageEnglish (US)
Pages (from-to)328-336
Number of pages9
JournalBiology of Reproduction
Volume80
Issue number2
DOIs
StatePublished - Feb 2009

Fingerprint

Ovary
Menopause
Messenger RNA
CD36 Antigens
LH Receptors
Hydroxysteroid Dehydrogenases
Proteins
Ovarian Follicle
Androstenedione
LDL Receptors
Mixed Function Oxygenases
4-vinyl-1-cyclohexene dioxide
Androgens
Fluorescent Antibody Technique
Public Health
Cholesterol
Staining and Labeling
Gene Expression

Keywords

  • 4-vinylcyclohexene diepoxide
  • Aging
  • Androgenesis
  • Interstitial cells
  • Ovarian failure
  • Ovary
  • Steroid hormones
  • Stroma
  • Theca cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Steroidogenic capacity of residual ovarian tissue in 4-vinylcyclohexene diepoxide- treated mice. / Rivera, Zelieann; Christian, Patricia J.; Marion, Sam L.; Brooks, Heddwen L; Hoyer, Patricia B.

In: Biology of Reproduction, Vol. 80, No. 2, 02.2009, p. 328-336.

Research output: Contribution to journalArticle

@article{9f2a854ca5464d589499474d841dd165,
title = "Steroidogenic capacity of residual ovarian tissue in 4-vinylcyclohexene diepoxide- treated mice",
abstract = "Menopause is an important public health issue because of its association with a number of disorders. Androgens produced by residual ovarian tissue after menopause could impact the development of these disorders. It has been unclear, however, whether the postmenopausal ovary retains steroidogenic capacity. Thus, an ovary-intact mouse model for menopause that uses the occupational chemical 4-vinylcyclohexene diepoxide (VCD) was used to characterize the expression of steroidogenic genes in residual ovarian tissue of follicle-depleted mice. Female B6C3F1 mice (age, 28 days) were dosed daily for 20 days with either vehicle or VCD (160 mg kg -1 day -1) to induce ovarian failure. Ovaries were collected on Day 181 and analyzed for mRNA and protein. Acyclic aged mice were used as controls for natural ovarian senescence. Relative to cycling controls, expression of mRNA encoding steroidogenic acute regulatory protein (Star); cholesterol side-chain cleavage (Cyp11a1); 3beta-hydroxysteroid dehydrogenase (Hsd3b); 17alpha-hydrox- ylase (Cyp17a1); scavenger receptor class B, type 1 (Scarbl); low-density lipoprotein receptor (Ldlr); and luteinizing hormone receptor (Lhcgr) was enriched in VCD-treated ovaries. In acyclic aged ovaries, mRNA expression for only Cyp17a1 and Lhcgr was greater than that in controls. Compared to cycling controls, ovaries from VCD-treated and aged mice had similar levels of HSD3B, CYP17A1, and LHCGR protein. The pattern of protein immunofluorescence staining for HSD3B in follicle- depleted (VCD-treated) ovaries was homogeneous, whereas that for CYP17A1 was only seen in residual interstitial cells. Circulating levels of FSH and LH were increased, and androstenedione levels were detectable following follicle depletion in VCD-treated mice. These findings support the idea that residual ovarian tissue in VCD-treated mice retains androgenic capacity.",
keywords = "4-vinylcyclohexene diepoxide, Aging, Androgenesis, Interstitial cells, Ovarian failure, Ovary, Steroid hormones, Stroma, Theca cells",
author = "Zelieann Rivera and Christian, {Patricia J.} and Marion, {Sam L.} and Brooks, {Heddwen L} and Hoyer, {Patricia B}",
year = "2009",
month = "2",
doi = "10.1095/biolreprod.108.070359",
language = "English (US)",
volume = "80",
pages = "328--336",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "2",

}

TY - JOUR

T1 - Steroidogenic capacity of residual ovarian tissue in 4-vinylcyclohexene diepoxide- treated mice

AU - Rivera, Zelieann

AU - Christian, Patricia J.

AU - Marion, Sam L.

AU - Brooks, Heddwen L

AU - Hoyer, Patricia B

PY - 2009/2

Y1 - 2009/2

N2 - Menopause is an important public health issue because of its association with a number of disorders. Androgens produced by residual ovarian tissue after menopause could impact the development of these disorders. It has been unclear, however, whether the postmenopausal ovary retains steroidogenic capacity. Thus, an ovary-intact mouse model for menopause that uses the occupational chemical 4-vinylcyclohexene diepoxide (VCD) was used to characterize the expression of steroidogenic genes in residual ovarian tissue of follicle-depleted mice. Female B6C3F1 mice (age, 28 days) were dosed daily for 20 days with either vehicle or VCD (160 mg kg -1 day -1) to induce ovarian failure. Ovaries were collected on Day 181 and analyzed for mRNA and protein. Acyclic aged mice were used as controls for natural ovarian senescence. Relative to cycling controls, expression of mRNA encoding steroidogenic acute regulatory protein (Star); cholesterol side-chain cleavage (Cyp11a1); 3beta-hydroxysteroid dehydrogenase (Hsd3b); 17alpha-hydrox- ylase (Cyp17a1); scavenger receptor class B, type 1 (Scarbl); low-density lipoprotein receptor (Ldlr); and luteinizing hormone receptor (Lhcgr) was enriched in VCD-treated ovaries. In acyclic aged ovaries, mRNA expression for only Cyp17a1 and Lhcgr was greater than that in controls. Compared to cycling controls, ovaries from VCD-treated and aged mice had similar levels of HSD3B, CYP17A1, and LHCGR protein. The pattern of protein immunofluorescence staining for HSD3B in follicle- depleted (VCD-treated) ovaries was homogeneous, whereas that for CYP17A1 was only seen in residual interstitial cells. Circulating levels of FSH and LH were increased, and androstenedione levels were detectable following follicle depletion in VCD-treated mice. These findings support the idea that residual ovarian tissue in VCD-treated mice retains androgenic capacity.

AB - Menopause is an important public health issue because of its association with a number of disorders. Androgens produced by residual ovarian tissue after menopause could impact the development of these disorders. It has been unclear, however, whether the postmenopausal ovary retains steroidogenic capacity. Thus, an ovary-intact mouse model for menopause that uses the occupational chemical 4-vinylcyclohexene diepoxide (VCD) was used to characterize the expression of steroidogenic genes in residual ovarian tissue of follicle-depleted mice. Female B6C3F1 mice (age, 28 days) were dosed daily for 20 days with either vehicle or VCD (160 mg kg -1 day -1) to induce ovarian failure. Ovaries were collected on Day 181 and analyzed for mRNA and protein. Acyclic aged mice were used as controls for natural ovarian senescence. Relative to cycling controls, expression of mRNA encoding steroidogenic acute regulatory protein (Star); cholesterol side-chain cleavage (Cyp11a1); 3beta-hydroxysteroid dehydrogenase (Hsd3b); 17alpha-hydrox- ylase (Cyp17a1); scavenger receptor class B, type 1 (Scarbl); low-density lipoprotein receptor (Ldlr); and luteinizing hormone receptor (Lhcgr) was enriched in VCD-treated ovaries. In acyclic aged ovaries, mRNA expression for only Cyp17a1 and Lhcgr was greater than that in controls. Compared to cycling controls, ovaries from VCD-treated and aged mice had similar levels of HSD3B, CYP17A1, and LHCGR protein. The pattern of protein immunofluorescence staining for HSD3B in follicle- depleted (VCD-treated) ovaries was homogeneous, whereas that for CYP17A1 was only seen in residual interstitial cells. Circulating levels of FSH and LH were increased, and androstenedione levels were detectable following follicle depletion in VCD-treated mice. These findings support the idea that residual ovarian tissue in VCD-treated mice retains androgenic capacity.

KW - 4-vinylcyclohexene diepoxide

KW - Aging

KW - Androgenesis

KW - Interstitial cells

KW - Ovarian failure

KW - Ovary

KW - Steroid hormones

KW - Stroma

KW - Theca cells

UR - http://www.scopus.com/inward/record.url?scp=59949096989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59949096989&partnerID=8YFLogxK

U2 - 10.1095/biolreprod.108.070359

DO - 10.1095/biolreprod.108.070359

M3 - Article

C2 - 18829706

AN - SCOPUS:59949096989

VL - 80

SP - 328

EP - 336

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 2

ER -