Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene

Kameha R. Kidd, Donny Dal Ponte, Alice L. Stone, James B. Hoying, Raymond B. Nagle, Stuart K. Williams

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Biomedical implants often exhibit poor clinical performance due to the formation of a periimplant avascular fibrous capsule. Surface modification of synthetic materials has been evaluated to accelerate the formation of functional microcirculation in association with implants. The current study used a flow-mediated protein deposition system to modify expanded polytetrafluoroethylene (ePTFE) with a laminin-5-rich conditioned growth medium and with medium from which laminin-5 had been selectively removed. An in vitro model of endothelial cell adherence determined that laminin-5 modification resulted in significantly increased adhesion of human micro vessel endothelial cells to ePTFE. In vivo studies evaluating the periimplant vascular response to laminin-5-treated samples indicated that absorption of laminin-5-rich conditioned medium supported accelerated neovascularization of ePTFE implants. A flow system designed to treat porous implant materials facilitates laminin-5 modification of commercially available ePTFE, resulting in increased endothelial cell adhesion in vitro and increased vascularization in vivo.

Original languageEnglish (US)
Pages (from-to)1379-1391
Number of pages13
JournalTissue Engineering
Volume11
Issue number9-10
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology

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