Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene

Kameha R. Kidd, Donny Dal Ponte, Alice L. Stone, James B. Hoying, Raymond B Nagle, Stuart K. Williams

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Biomedical implants often exhibit poor clinical performance due to the formation of a periimplant avascular fibrous capsule. Surface modification of synthetic materials has been evaluated to accelerate the formation of functional microcirculation in association with implants. The current study used a flow-mediated protein deposition system to modify expanded polytetrafluoroethylene (ePTFE) with a laminin-5-rich conditioned growth medium and with medium from which laminin-5 had been selectively removed. An in vitro model of endothelial cell adherence determined that laminin-5 modification resulted in significantly increased adhesion of human micro vessel endothelial cells to ePTFE. In vivo studies evaluating the periimplant vascular response to laminin-5-treated samples indicated that absorption of laminin-5-rich conditioned medium supported accelerated neovascularization of ePTFE implants. A flow system designed to treat porous implant materials facilitates laminin-5 modification of commercially available ePTFE, resulting in increased endothelial cell adhesion in vitro and increased vascularization in vivo.

Original languageEnglish (US)
Pages (from-to)1379-1391
Number of pages13
JournalTissue Engineering
Volume11
Issue number9-10
DOIs
StatePublished - Sep 2005

Fingerprint

Cell adhesion
Endothelial cells
Polytetrafluoroethylene
Polytetrafluoroethylenes
Cell Adhesion
Endothelial Cells
Conditioned Culture Medium
Microcirculation
Surface treatment
Adhesion
Proteins
Capsules
Blood Vessels
kalinin
Growth

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene. / Kidd, Kameha R.; Dal Ponte, Donny; Stone, Alice L.; Hoying, James B.; Nagle, Raymond B; Williams, Stuart K.

In: Tissue Engineering, Vol. 11, No. 9-10, 09.2005, p. 1379-1391.

Research output: Contribution to journalArticle

Kidd, Kameha R. ; Dal Ponte, Donny ; Stone, Alice L. ; Hoying, James B. ; Nagle, Raymond B ; Williams, Stuart K. / Stimulated endothelial cell adhesion and angiogenesis with laminin-5 modification of expanded polytetrafluoroethylene. In: Tissue Engineering. 2005 ; Vol. 11, No. 9-10. pp. 1379-1391.
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