Stimulated Raman scattering microscopy with a robust fibre laser source

Christian W. Freudiger, Wenlong Yang, Gary R. Holtom, Nasser N Peyghambarian, X. Sunney Xie, Khanh Q Kieu

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame s -1.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalNature Photonics
Volume8
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Stimulated Raman scattering
Fiber lasers
fiber lasers
Microscopic examination
Raman spectra
microscopy
Imaging techniques
Raman scattering
Shot noise
image contrast
shot noise
Materials science
materials science
power amplifiers
Power amplifiers
biology
medicine
subtraction
cancellation
Medicine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Stimulated Raman scattering microscopy with a robust fibre laser source. / Freudiger, Christian W.; Yang, Wenlong; Holtom, Gary R.; Peyghambarian, Nasser N; Xie, X. Sunney; Kieu, Khanh Q.

In: Nature Photonics, Vol. 8, No. 2, 02.2014, p. 153-159.

Research output: Contribution to journalArticle

Freudiger, Christian W. ; Yang, Wenlong ; Holtom, Gary R. ; Peyghambarian, Nasser N ; Xie, X. Sunney ; Kieu, Khanh Q. / Stimulated Raman scattering microscopy with a robust fibre laser source. In: Nature Photonics. 2014 ; Vol. 8, No. 2. pp. 153-159.
@article{7eafa0bc21b14117b6c52da5a95597c4,
title = "Stimulated Raman scattering microscopy with a robust fibre laser source",
abstract = "Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame s -1.",
author = "Freudiger, {Christian W.} and Wenlong Yang and Holtom, {Gary R.} and Peyghambarian, {Nasser N} and Xie, {X. Sunney} and Kieu, {Khanh Q}",
year = "2014",
month = "2",
doi = "10.1038/nphoton.2013.360",
language = "English (US)",
volume = "8",
pages = "153--159",
journal = "Nature Photonics",
issn = "1749-4885",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Stimulated Raman scattering microscopy with a robust fibre laser source

AU - Freudiger, Christian W.

AU - Yang, Wenlong

AU - Holtom, Gary R.

AU - Peyghambarian, Nasser N

AU - Xie, X. Sunney

AU - Kieu, Khanh Q

PY - 2014/2

Y1 - 2014/2

N2 - Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame s -1.

AB - Stimulated Raman scattering microscopy allows label-free chemical imaging and has enabled exciting applications in biology, material science and medicine. It provides a major advantage in imaging speed over spontaneous Raman scattering and has improved image contrast and spectral fidelity compared to coherent anti-Stokes Raman scattering. Wider adoption of the technique has, however, been hindered by the need for a costly and environmentally sensitive tunable ultrafast dual-wavelength source. We present the development of an optimized all-fibre laser system based on the optical synchronization of two picosecond power amplifiers. To circumvent the high-frequency laser noise intrinsic to amplified fibre lasers, we have further developed a high-speed noise cancellation system based on voltage-subtraction autobalanced detection. We demonstrate uncompromised imaging performance of our fibre-laser-based stimulated Raman scattering microscope with shot-noise-limited sensitivity and an imaging speed up to 1 frame s -1.

UR - http://www.scopus.com/inward/record.url?scp=84895059717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84895059717&partnerID=8YFLogxK

U2 - 10.1038/nphoton.2013.360

DO - 10.1038/nphoton.2013.360

M3 - Article

VL - 8

SP - 153

EP - 159

JO - Nature Photonics

JF - Nature Photonics

SN - 1749-4885

IS - 2

ER -