Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus

S. Kajimura, K. Uchida, T. Yada, L. G. Riley, J. C. Byatt, Robert J Collier, K. Aida, T. Hirano, E. G. Grau

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We have previously reported growth-promoting effects of recombinant bovine growth hormone (rbGH) in Mozambique tilapia, Oreochromis mossambicus, after 4 weekly injections or a single injection of slow-releasing formulation (Posilac®) (Leedom et al. 2002). In order to obtain further understanding of the role of the growth hormone (GH)-insulin-like growth factor-I (IGF-I) axis in growth in the tilapia, the effects of rbGH on plasma and mRNA levels of IGF-I were examined. Plasma IGF-I levels were significantly increased after rbGH and Posilac® injections, and a significant correlation was observed between plasma IGF-I levels, body length and mass in both treatments. IGF-I mRNA levels in the liver and in the skeletal muscle were also significantly increased after rbGH and Posilac® injections, indicating that IGF-I gene expression in these tissues is under control of circulating GH. IGF-I mRNA levels in the gill were not affected by treatment. Liver IGF-I mRNA levels were significantly correlated with body length and with body mass after rbGH and Posilac® injections. These results indicate that the growth-promoting effect of rbGH in this species is mediated to a significant extent via its stimulation of hepatic production of IGF-I and the resulting increase in plasma IGF-I, and also possibly through locally produced IGF-I in the skeletal muscle, acting in a paracrine or autocrine fashion.

Original languageEnglish (US)
Pages (from-to)221-230
Number of pages10
JournalFish Physiology and Biochemistry
Volume25
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Tilapia
Oreochromis mossambicus
insulin-like growth factor I
Insulin-Like Growth Factor I
somatotropin
Growth Hormone
hormone
injection
Injections
Plasmas
Messenger RNA
Liver
body length
liver
Muscle
skeletal muscle
Skeletal Muscle
bovine growth hormone
bovine
Growth

Keywords

  • Growth hormone
  • Insulin-like growth factor-I
  • Oreochromis mossambicus
  • RNase protection assay
  • Tilapia

ASJC Scopus subject areas

  • Aquatic Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Physiology
  • Physiology (medical)

Cite this

Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus. / Kajimura, S.; Uchida, K.; Yada, T.; Riley, L. G.; Byatt, J. C.; Collier, Robert J; Aida, K.; Hirano, T.; Grau, E. G.

In: Fish Physiology and Biochemistry, Vol. 25, No. 3, 2002, p. 221-230.

Research output: Contribution to journalArticle

Kajimura, S. ; Uchida, K. ; Yada, T. ; Riley, L. G. ; Byatt, J. C. ; Collier, Robert J ; Aida, K. ; Hirano, T. ; Grau, E. G. / Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus. In: Fish Physiology and Biochemistry. 2002 ; Vol. 25, No. 3. pp. 221-230.
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