Storage protein accumulation patterns in somatic embryos of cotton (Gossypium hirsutum L.)

R. C. Shoemaker, S. E. Christofferson, D. W. Galbraith

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The storage protein content of somatic embryos of Gossypium hirsutum L. cv. Coker 201 was determined using extinction level, antigen/antibody association detection methods. Mature storage protein was first detected in early globular-stage somatic embryos at a total concentration of 0.36% of the embryo protein mass. Tulip-stage and mature somatic embryos were comprised of 3.0% and 1.3% mature storage protein, respectively. Maximum storage protein synthesis was found to occur during early globular- and early heart-stages. During this period of development, significant levels of protein precursors were found also to accumulate. The pattern of storage protein synthesis, processing and accumulation paralleled the pattern that has been reported for the zygotic system, although somatic embryos accumulate storage protein at much earlier stages and to a lesser degree. The possibility of using complex biochemical pathways to monitor embryogenic systems in vitro is discussed.

Original languageEnglish (US)
Pages (from-to)12-15
Number of pages4
JournalPlant Cell Reports
Volume6
Issue number1
DOIs
StatePublished - Feb 1 1987
Externally publishedYes

Keywords

  • Cotton
  • Gossypium hirsutum L.
  • Somatic embryogenesis
  • storage proteins

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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