Streptozotocin (STZ) diabetes enhances benzo(α)pyrene induced renal injury in Sprague Dawley rats

Monica A. Valentovic, Napoleon Alejandro, A. Betts Carpenter, Patrick I. Brown, Kenneth Ramos

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Information is lacking regarding the biological response to environmental chemicals in the context of pre-existing disease. Benzo(α)pyrene (BaP), a polycyclic aromatic hydrocarbon, is a byproduct of combustion that causes renal injury and elicits a nephropathic response. This study evaluated the nephrotoxicity of BaP in normoglycemic and diabetic rats. Female Sprague Dawley rats were divided into four groups: normoglycemic-vehicle (NV), normoglycemic-BaP (N-BaP), diabetic-vehicle (DV) and diabetic-BaP (D-BaP). Diabetes was induced by intraperitoneal (ip) injection of streptozotocin (60 mg/kg, 1 ml/kg). Rats were injected (ip) with vehicle or 10 mg/kg BaP (1 ml/kg) once per week for 5 weeks. Urinary protein and albumin, plasma creatinine and light microscopy were performed to assess the effects of BaP on kidney function. Diabetes was confirmed by plasma glucose levels >400 mg/dl in the DV and D-BaP groups. BaP increased kidney weight and blood urea nitrogen (BUN) levels in the D-BaP relative to the DV group. No change in BUN was observed following 5 weeks of BaP treatment in the normoglycemic animals, however, kidney weight was increased (p = 0.013) in the N-BaP relative to the NV animals. STZ diabetes increased susceptibility to BaP mediated renal damage following repeated treatment for 5 weeks when compared to age matched normoglycemic rats.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalToxicology letters
Volume164
Issue number3
DOIs
StatePublished - Jul 15 2006
Externally publishedYes

Keywords

  • Benzo(α)pyrene
  • Diabetic nephropathy
  • Microalbuminuria
  • Nephrotoxicity

ASJC Scopus subject areas

  • Toxicology

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