Stress-specific composition, assembly and kinetics of stress granules in Saccharomyces cerevisiae

John R Buchan, Je Hyun Yoon, Roy Parker

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Eukaryotic cells respond to cellular stresses by the inhibition of translation and the accumulation of mRNAs in cytoplasmic RNA-protein (ribonucleoprotein) granules termed stress granules and P-bodies. An unresolved issue is how different stresses affect formation of messenger RNP (mRNP) granules. In the present study, we examine how sodium azide (NaN3), which inhibits mitochondrial respiration, affects formation of mRNP granules as compared with glucose deprivation in budding yeast. We observed that NaN 3 treatment inhibits translation and triggers formation of P-bodies and stress granules. The composition of stress granules induced by NaN 3 differs from that of glucose-deprived cells by containing eukaryotic initiation factor (eIF)3, eIF4A/B, eIF5B and eIF1A proteins, and by lacking the heterogeneous nuclear RNP (hnRNP) protein Hrp1. Moreover, in contrast with glucose-deprived stress granules, NaN3-triggered stress granules show different assembly rules, form faster and independently from P-bodies and dock or merge with P-bodies over time. Strikingly, addition of NaN3 and glucose deprivation in combination, regardless of the order, always results in stress granules of a glucose deprivation nature, suggesting that both granules share an mRNP remodeling pathway. These results indicate that stress granule assembly, kinetics and composition in yeast can vary in a stress-specific manner, which we suggest reflects different rate-limiting steps in a common mRNP remodeling pathway.

Original languageEnglish (US)
Pages (from-to)228-239
Number of pages12
JournalJournal of Cell Science
Volume124
Issue number2
DOIs
StatePublished - Jan 15 2011

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Sodium Azide
Saccharomyces cerevisiae
Glucose
Eukaryotic Initiation Factor-3
Saccharomycetales
Ribonucleoproteins
Protein Biosynthesis
Eukaryotic Cells
Nuclear Proteins
Respiration
Proteins
Yeasts
RNA

Keywords

  • mRNA
  • P-bodies
  • Stress granules
  • Translation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Stress-specific composition, assembly and kinetics of stress granules in Saccharomyces cerevisiae. / Buchan, John R; Yoon, Je Hyun; Parker, Roy.

In: Journal of Cell Science, Vol. 124, No. 2, 15.01.2011, p. 228-239.

Research output: Contribution to journalArticle

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