Stressed apoptotic tumor cells stimulate dendritic cells and induce specific cytotoxic T cells

Hanping Feng, Yi - Zeng, Michael W. Graner, Emmanuel Katsanis

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

We have previously reported that stressed apoptotic tumor cells are more immunogenic in vivo than nonstressed ones. Using confocal microscopy we have confirmed our previous observation that heat-stressed apoptotic 12B1-D1 leukemia cells (BCR-ABL+) express HSP60 and HSP72 on their surface. To explore how the immune system distinguishes stressed from nonstressed apoptotic tumor cells, we analyzed the responses of dendritic cells to these 2 types of apoptotic cells. We found that nonstressed and heat-stressed apoptotic 12B1-D1 cells were taken up by dendritic cells in a comparable fashion. However, when stressed apoptotic 12B1-D1 cells were coincubated with immature dendritic cells for 24 hours, this resulted in greater up-regulation of co-stimulatory molecules (CD40, CD80, and CD86) on the surface of dendritic cells. Moreover, stressed apoptotic 12B1-D1 cells were more effective in stimulating dendritic cells to secrete interleukin-12 (IL-12) and in enhancing their immuno-stimulatory functions in mixed leukocyte reactions. Furthermore, we demonstrated that immunization of mice with stressed apoptotic 12B1-D1 cells induced the secretion of T helper-1 (TH1) profile of cytokines by spleen cells. Splenocytes from mice immunized with stressed apoptotic cells, but not nonstressed ones, were capable of lysing 12B1-D1 and the parental 12B1 line, but not a B-cell leukemia line, A20. Our data indicate that stressed apoptotic tumor cells are capable of providing the necessary danger signals, likely through increased surface expression of heat shock proteins (HSPs), resulting in activation/maturation of dendritic cells and, ultimately, the generation of potent antitumor T-cell responses.

Original languageEnglish (US)
Pages (from-to)4108-4115
Number of pages8
JournalBlood
Volume100
Issue number12
DOIs
StatePublished - Dec 1 2002

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T-cells
Dendritic Cells
Tumors
Cells
T-Lymphocytes
Neoplasms
Immunization
Confocal microscopy
Immune system
Interleukin-12
Heat-Shock Proteins
Hot Temperature
B-Cell Leukemia
Chemical activation
Cytokines
Mixed Lymphocyte Culture Test
Molecules
Confocal Microscopy
Immune System
Leukemia

ASJC Scopus subject areas

  • Hematology

Cite this

Stressed apoptotic tumor cells stimulate dendritic cells and induce specific cytotoxic T cells. / Feng, Hanping; Zeng, Yi -; Graner, Michael W.; Katsanis, Emmanuel.

In: Blood, Vol. 100, No. 12, 01.12.2002, p. 4108-4115.

Research output: Contribution to journalArticle

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