Structural and functional analyses of mutant fur proteins with impaired regulatory function

A. M. Wertheimer, M. E. Tolmasky, L. A. Actis, J. H. Crosa

Research output: Contribution to journalComment/debate

25 Scopus citations

Abstract

Vibrio anguillarum Fur mutants, 775met9 and 775met11, were characterized. V. anguillarum 775met9 had a change of D to G at position 104 located in the carboxy terminus resulting in impaired Fur activity. Computer analysis predicts perturbation of an alpha-helix in the carboxy terminus which may interfere with Fur protein conformation. Strain 775met11 had a change in the start codon resulting in no protein synthesis. The mutants are unstable, and reversion to the wild type occurs frequently.

Original languageEnglish (US)
Pages (from-to)5116-5122
Number of pages7
JournalJournal of bacteriology
Volume176
Issue number16
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Structural and functional analyses of mutant fur proteins with impaired regulatory function'. Together they form a unique fingerprint.

  • Cite this