Structural changes of tailless bacteriophage Φx174 during penetration of bacterial cell walls

Yingyuan Sun, Aaron P. Roznowski, Joshua M. Tokuda, Thomas Klose, Alexander Mauney, Lois Pollack, Bentley A Fane, Michael G. Rossmann

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection states. The ejection is initiated by the loss of the G protein spike that encounters the LPS, followed by conformational changes of two polypeptide loops on the major capsid F proteins. One of these loops mediates viral attachment, and the other participates in making the fivefold channel at the vertex contacting the LPS.

Original languageEnglish (US)
Pages (from-to)13708-13713
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number52
DOIs
StatePublished - Dec 26 2017

Fingerprint

Bacteriophages
Cell Wall
Lipopolysaccharides
Genome
Cryoelectron Microscopy
Single-Stranded DNA
Capsid Proteins
GTP-Binding Proteins
X-Rays
Bacteria
Peptides

Keywords

  • Bacteriophage
  • Cryo-EM
  • DNA ejection
  • Lipopolysaccharide
  • ΦX174

ASJC Scopus subject areas

  • General

Cite this

Structural changes of tailless bacteriophage Φx174 during penetration of bacterial cell walls. / Sun, Yingyuan; Roznowski, Aaron P.; Tokuda, Joshua M.; Klose, Thomas; Mauney, Alexander; Pollack, Lois; Fane, Bentley A; Rossmann, Michael G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 52, 26.12.2017, p. 13708-13713.

Research output: Contribution to journalArticle

Sun, Yingyuan ; Roznowski, Aaron P. ; Tokuda, Joshua M. ; Klose, Thomas ; Mauney, Alexander ; Pollack, Lois ; Fane, Bentley A ; Rossmann, Michael G. / Structural changes of tailless bacteriophage Φx174 during penetration of bacterial cell walls. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 52. pp. 13708-13713.
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