Structural study of the H/ACA snoRNP components Nop10p and the 3′ hairpin of U65 snoRNA

May Khanna, W. U. Haihong, Carina Johansson, Michèle Caizergues-Ferrer, Juli Feigon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the modification of uridine to pseudouridine at conserved sites in rRNA. The H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We have determined the solution structure of an RNA hairpin derived from the human U65 box H/ACA snoRNA including the pseudouridylation pocket and adjacent stems, providing the first three-dimensional structural information on these H/ACA snoRNAs. We have also determined the structure of Nop10p and investigated its interaction with RNA using NMR spectroscopy. Nop10p contains a structurally well-defined N-terminal region composed of a β-hairpin, and the rest of the protein lacks a globular structure. Chemical shift mapping of the interaction of RNA constructs of U65 box H/ACA 3′ hairpin with Nop10p shows that the β-hairpin binds weakly but specifically to RNA. The unstructured region of Nop10p likely interacts with Cbf5p. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)40-52
Number of pages13
JournalRNA
Volume12
Issue number1
DOIs
StatePublished - Jan 2006
Externally publishedYes

Fingerprint

Small Nucleolar Ribonucleoproteins
Small Nucleolar RNA
RNA
Pseudouridine
Uridine
Proteins
Magnetic Resonance Spectroscopy
Yeasts

Keywords

  • β-hairpin
  • NMR
  • Pseudouridylation
  • RNA-protein

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Khanna, M., Haihong, W. U., Johansson, C., Caizergues-Ferrer, M., & Feigon, J. (2006). Structural study of the H/ACA snoRNP components Nop10p and the 3′ hairpin of U65 snoRNA. RNA, 12(1), 40-52. https://doi.org/10.1261/rna.2221606

Structural study of the H/ACA snoRNP components Nop10p and the 3′ hairpin of U65 snoRNA. / Khanna, May; Haihong, W. U.; Johansson, Carina; Caizergues-Ferrer, Michèle; Feigon, Juli.

In: RNA, Vol. 12, No. 1, 01.2006, p. 40-52.

Research output: Contribution to journalArticle

Khanna, M, Haihong, WU, Johansson, C, Caizergues-Ferrer, M & Feigon, J 2006, 'Structural study of the H/ACA snoRNP components Nop10p and the 3′ hairpin of U65 snoRNA', RNA, vol. 12, no. 1, pp. 40-52. https://doi.org/10.1261/rna.2221606
Khanna, May ; Haihong, W. U. ; Johansson, Carina ; Caizergues-Ferrer, Michèle ; Feigon, Juli. / Structural study of the H/ACA snoRNP components Nop10p and the 3′ hairpin of U65 snoRNA. In: RNA. 2006 ; Vol. 12, No. 1. pp. 40-52.
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