Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength

Adnan Iqbal, Ayesha Latif, David W. Galbraith, Basit Jabbar, Muhmmad Azam Ali, Mukhtar Ahmed, Ambreen Gul, Abdul Qayyum Rao, Ahmad Ali Shahid, Tayyab Husnain

Research output: Contribution to journalArticlepeer-review

Abstract

Cotton fibre quality is a multigenic trait. Genetic modification of different genes to achieve high quality fibre is difficult without knowing the mechanism lying behind genes interaction. Based on background knowledge an attempt to explore the potential structural interactions between Gossypium hirsutum Wlim5 domain1 and Gossypium hirsutum ACTIN-1 proteins was done in current study. Sequence features of the LIM domain1 of GhWlim5 protein were identified through multiple sequence alignment analysis, and a phylogenetic tree was built to identify evolutionary relationships between sequences. Conservation indicated the evolutionary importance of side chain residues and the presence of several aliphatic and/or bulky residues, which stabilize the protein core and facilitate packing of zinc fingers. The structures of GhWlim5 domain1 and GhACTIN-1 proteins were modelled and validated through computational methods. Validation of GhACTIN-1 and GhWlim5 domain1 structures indicated good structural quality with 99.7% and 100% of the favoured number of residues in allowed regions and Z-score, within the ranges of − 9.87 and − 4.17, respectively. Docking analysis indicated various possible modes of interaction between these two proteins with favourable binding affinities. Based on our strong binding interaction results between GhWlim5 domain1 and GhACTIN-1 proteins, we further investigated the role of over-expression of GhWlim5 by transformation in cotton plants under fibre specific promoter and transgenic plants displayed significant increases in fibre strength.

Original languageEnglish (US)
Pages (from-to)373-386
Number of pages14
JournalJournal of Plant Biochemistry and Biotechnology
Volume30
Issue number2
DOIs
StatePublished - Jun 2021

Keywords

  • Cotton fibre
  • GhWlim5 protein
  • Gossypium hirsutism ACTIN-1 (GhACTIN-1) protein
  • Seed coat and fiber-specific protease (GhSCFP)
  • Structural interactions

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Fingerprint

Dive into the research topics of 'Structure-based prediction of protein–protein interactions between GhWlim5 Domain1 and GhACTIN-1 proteins: a practical evidence with improved fibre strength'. Together they form a unique fingerprint.

Cite this